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| Thomaz-Soccol,Andréa; Mocellin,Marcos; Mulinari,Fabiane; Castro,Edilene Alcântara de; Queiroz-Telles,Flávio de; Alcântara,Fernanda de Souza; Bavaresco,Marcello Tortelli; Hennig,Luciane; Andraus,Anielo; Luz,Ennio; Thomaz-Soccol,Vanete. |
| The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucocutaneous leishmaniasis; Molecular diagnosis; Leishmania (Viannia) braziliensis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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| Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio. |
| Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012 |
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| Szargiki,Regiane; Castro,Edilene Alcântara de; Luz,Ennio; Kowalthuk,Wolodymir; Machado,Ângela Maria; Thomaz-Soccol,Vanete. |
| We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutaneous leishmaniasis; Immunodiagnosis; Antigen; Western blotting. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011 |
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