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Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene Genet. Mol. Biol.
Wang,Honggang; Sun,Wuzhuang; Li,Zhu; Wang,Xiufang; Lv,Zhanjun.
Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence characteristics of these elements. We found that sense and antisense PolyA of simian virus 40 (SV40PolyA, 240 bp) eliminated the repression of GFP gene expression when inserted between the GFP gene and the Alu (283 bp) repeats or ORF2 (3825 bp) in pAlu14 (14 tandem Alu repeats were inserted downstream of the GFP gene in the vector pEGFP-C1) or pORF2. Antisense SV40PolyA (PolyAas) induced stronger gene expression than its sense orientation (PolyA). Of four 60-bp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alu; Enhancer; GFP; Stem-loop structures; SV40PolyA.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300005
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