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| John,Ana Laura de Wallau; Mäder,Geraldo; Fregonezi,Jeferson N.; Freitas,Loreta B.. |
| Abstract Calibrachoa is a South-American genus comprising 27 species, several considered endemic or rare; few were subjects in genetic studies. We attempted to generate new data about the phylogenetically related and rare species C. eglandulata, C. sendtneriana, C. serrulata, and C. spathulata concerning their genetic diversity and population structure, which, coupled with their known restricted distribution, could help access their conservation status and contribute to the study of the Brazilian biodiversity. We sequenced 88 individuals for plastid intergenic spacers and genotyped 186 individuals for five microsatellite loci. Compared among each other, C. sendtneriana and C. serrulata presented the highest values of genetic diversity [π% (sd) = 0.23... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plastid DNA; Microsatellites; Threatened species; Grasslands; Herbaceous species. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100108 |
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| Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de. |
| Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135 |
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| Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B.. |
| The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
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