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| Registros recuperados: 93 | |
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| GONÇALVES, A. P.; FREITAS, A. T.; KUWAHARA, M. K.; MARCELINO-GUIMARÃES, F. C.; ALMEIDA, A. M. R.; BIANCHINI, A.; MOLINA, R. O.. |
| A cultura do feijoeiro (Phaseolus vulgaris L.) é afetada pela virose conhecida como mosaico dourado, Bean golden mosaic virus (BGMV), é transmitido por mosca-branca [Bemisia tabaci (Genn.)]. Objetivo foi detectar e quantificar a presença do BGMV em diferentes linhagens de feijão e em Calopogônio (Calopogonio mucunoides). O experimento foi conduzido em ambiente controlado, inteiramente casualizado com quatro repetições. As plantas foram inoculadas com isolados sintomáticos para BGMV, por moscas-branca virulíferas, no período de 48 horas. As plantas foram avaliadas para sintomas no período de 7,10, 15, 20 e 25 dias após a inoculação, seguido da coleta de folhas para extração total de DNA. As reações de qPCR foram conduzidas com os 0,4uM de iniciadores 2371 e... |
| Tipo: Parte de livro |
Palavras-chave: Fitopatologia; BGMV; PCR-Quantitativo.; Feijão; Mosaico Dourado.; Doença de Planta; Vírus; Bean golden mosaic virus.; Plant diseases and disorders; Beans. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025581 |
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| KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R.. |
| Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and... |
| Tipo: Separatas |
Palavras-chave: Soja; Deficiência hídrica; Gene marcador; Soybeans; Gene expression; Soil water deficit. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/911802 |
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| GIROTTO, L.; SOLDERA, M. C. A.; HONNA, P. T.; KANAMORI, N.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.. |
| A seca é, atualmente, um importante fator responsável por perdas na produção brasileira de soja. A busca por cultivares adaptadas a esta condição pode ser realizada através do melhoramento genético, pela inserção de genes por diferentes métodos de transformação de plantas, entre outros. Sucessos obtidos com a transformação de Arabdopsis thaliana com gene AREB1 evidenciou importantes respostas das plantas à seca e a alta salinidade. Visando a obtenção de genótipos de soja geneticamente modificada para tolerância à seca e a alta salinidade, este trabalho teve como objetivo inserir a construção 35S:AREB1 em cultivares de soja BR 16 pela técnica de transformação via Agrobacterium tumefaciens. Neste trabalho foram transformados 8731 embriões imaturos, dos quais... |
| Tipo: Parte de livro |
Palavras-chave: Biotecnologia.. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/928157 |
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| SANTOS, I, M. O. dos; ABE, V. Y.; CARVALHO, K. de; BARAZETTI, A. R.; SIMIONATO, A. S.; PEGA, G. E. de A.; MATIS, S. H.; CANO, B. G.; CELY, M. V. T.; MARCELINO-GUIMARÃES, F. C.; CHRYSSAFIDS, A. L.; ANDRADE, G.. |
| Tipo: Artigo de periódico |
Palavras-chave: Ferrugem; Soja; Doença de Planta; Rust diseases; Soybean rust; Bioactive compounds. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133222 |
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| LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; LUANA M. DARBEN; KUWAHARA, M. K.; NEPOMUCENO, A. L.; DIAS, W. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.. |
| The Hsp20 genes are associated with stress caused by HS and other abiotic factors, but have recently been found to be associated with the response to biotic stresses. These genes represent the most abundant class among the HSPs in plants, but little is known about this gene family in soybean. Because of their apparent multifunctionality, these proteins are promising targets for developing crop varieties that are better adapted to biotic and abiotic stresses. Thus, in the present study an in silico identification of GmHsp20 gene family members was performed, and the genes were characterized and subjected to in vivo expression analysis under biotic and abiotic stresses. A search of the available soybean genome databases revealed 51 gene models as potential... |
| Tipo: Separatas |
Palavras-chave: Soja.. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/965345 |
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| SOARES, R. M.; FANTINATO, G. G. P.; DARBEN, L. M.; MARCELINO-GUIMARÃES, F. C.; SEIXAS, C. D. S.; CARNEIRO, G. E. de S.. |
| Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) causes bacterial wilt on beans (Phaseolus vulgaris) and bacterial tan spot on soybeans (Glycine max). Cff was detected on beans in Brazil in 1995. Plants of commercial and experimental fields of soybean with typical symptoms of the disease were collected in the State of Paraná, Brazil, during the 2011/2012 growing season. The causal agent was identified as Cff by isolation from symptomatic leaves on CNS semi-selective medium, artificial inoculation test and re-isolation in soybean and bean, Gram staining test, solubility in KOH, and by PCR. This is the first report of Cff on soybean in Brazil. |
| Tipo: Artigo de periódico |
Palavras-chave: Soja; Doença de planta; Bactéria; Soybeans; Plant diseases and disorders; Curtobacterium flaccumfaciens pv. flaccumfaciens. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/972597 |
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| CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.. |
| The soybean rust caused by the fungus Phakopsora pachyrhizi, has caused serious damage to soybean culture since 2001 in Brazil. Several sources of rust resistance have already been identified; however no cultivar has shown stable resistance to rust. Plants are commonly infected by one or more strains of the fungus causing the breakdown of resistance acquired with the introduction of only one or a few resistance genes. In the last years, different study strategies have allowed the simultaneous monitoring of gene expression in plant-pathogen interaction, broadening our understanding of the molecular mechanisms underlying compatibility and incompatibility responses of soybean to P. pachyrhizi creating new perspectives for the development of a more durable... |
| Tipo: Parte de livro |
Palavras-chave: Soja; Doença de Planta; Ferrugem.; Plant diseases and disorders; Soybeans; Soybean rust.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010927 |
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| BÜCKER NETO, L.; OLIVEIRA, R. R. de; WIEBKE-STROHM, B.; BENCKE, M.; WEBER, R. L. M.; CABREIRA, C.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; ZANETTINI, M. H. D.; PASSAGLIA, L. M. P.. |
| Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in... |
| Tipo: Artigo de periódico |
Palavras-chave: Ferrugem asiática da soja. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/986708 |
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| BRITTO-KIDO, S. de A.; FERREIRA NETO, J. R. C.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; BENKO-ISEPPON, A. M.; KIDO, E. A.. |
| Natural antisense ranscripts (NAT) are RNA molecules complementary to other endogenous RNAs. They are capable of regulating the expression of target genes at different levels (transcription, mRNA stability, translation, etc.). Such a property makes them ideal for interventions in organisms? metabolism. The present study reviewed plant NAT aspects, including features, availability and genesis, conservation and distribution, coding capacity, NAT pair expression, and functions. Besides, an in silico identification of NATs pairs was presented, using deepSuperSAGE libraries of soybean infected or not with Phakopsora pachyrhizi.Resultsshowed that around 1/3 of the 77,903 predicted trans-NATs (by PlantsNATsDB database) detected had unitags mapped in both... |
| Tipo: Separatas |
Palavras-chave: Soja.. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/962186 |
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| BASSO, M. F.; LOURENCO, I. T.; MOREIRA-PINTO, C. E.; MENDES, R. A. G.; PAES-DE-MELO, B.; NEVES, M. R. das; MACEDO, A. F.; FIGUEIREDO, V.; GRANDIS, A.; MACEDO, L. L. P. de; ARRAES, F. B. M.; COSTA, M. M. do C.; TOGAWA, R. C.; ENRICH-PRAST, A.; MARCELINO-GUIMARÃES, F. C.; GOMES, A. C. M. M.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. |
| Na publicação: Isabela Tristan Lourenço-Tessutti; Leonardo Lima Pepino Macedo; Francismar Corrêa Marcelino-Guimaraes; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
| Tipo: Artigo de periódico |
Palavras-chave: Glyma 11G121800; New biotechnology tools; Phytoglobins; Plant-nematode interaction; Rootknot nematodes; PI595099; BRS133; Glycine Max; Soja; Nematóide; Soybeans; Nematoda. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151081 |
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| MORALES, A.; PEREIRA, A. A.; CATELLI, L.; BORÉM, A.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, M. C. N. de; MICHELLE A. GRAHAM; HOFFMANN-CAMPO, C. B.; ABDELNOOR, R. V.. |
| Asian soybean rust (ASR) is a soybean disease caused by Phakopsora pachyrhizi Sydow. The phenylpropanoid pathway is involved in many biological processes including the defense response to the fungus. This pathway results in the production of lignin and phytoalexins, which are an important defense against pathogens and insects. In the present work, we used high performance liquid chromatography (HPLC) to measure the production of compounds in the phenylpropanoid pathway in leaves of the resistant soybean genotype (PI459025B, Rpp4). Plants were either infected with P. pachyrhizi or mock infected with water, and leaf tissue was collected at 24, 48, 72, 96, 120, 161, 308 and 504 hours after inoculation (hai). Phenolic peak quantification was carried out by... |
| Tipo: Separatas |
Palavras-chave: Soja; Doença de planta; Ferrugem; Phakopsora Pachyrhizi.; Soybeans; Plant diseases and disorders; Rust diseases.. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940515 |
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| SANTOS, J. V. M. dos; JOSHI, T.; KHAN, S. M.; LIU, Y.; WANG, J.; VUONG, T. D.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, M. F. de; VALLIYODAN, B.; XU, D.; NGUYEN, H. T.; ABDELNOOR, R. V.. |
| Tipo: Parte de livro |
Palavras-chave: Soja; Genoma; Melhoramento genético vegetal; Soybeans; Genomics. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1018778 |
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| Registros recuperados: 93 | |
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