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| Registros recuperados: 42 | |
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| KAWASHIMA, Chiho; KANEKO, Etsushi; AMAYA MONTOYA, Carlos; MATSUI, Motozumi; YAMAGISHI, Norio; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; KIDA, Katsuya; MIYAKE, Yoichi; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 石井, 三都夫; 木田, 克弥; 三宅, 陽一; 宮本, 明夫. |
| The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous... |
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Palavras-chave: Dairy cow; First ovulation; Postpartum; Resumption of ovarian cycle. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/916 |
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| LEE, Hsu-Hsun; KIDA, Katsuya; MIURA, Ryotaro; INOKUMA, Hisashi; MIYAMOTO, Akio; KAWASHIMA, Chiho; HANEDA, Shingo; MIYAKE, Yoh-Ichi; MATSUI, Motozumi; 猪熊, 壽; 宮本, 明夫; 川島, 千帆; 羽田, 真悟; 松井, 基純. |
| In peripartum dairy cows, insulin resistance (IR) increases to adjust the direction of energy to lactation after calving. To investigate the effect of prepartum IR on postpartum reproductive performance, the insulin tolerance test (ITT) was applied to 15 cows at 3 weeks (Pre21) and 10 days (Pre10) before the predicted calving date. Blood glucose area under the curve (AUCglu) within 120 min after administration of 0.05 IU/kg-BW insulin was calculated. The occurrence of first ovulation, days to first artificial insemination (AI) and first AI conception rate were recorded. Nutritional status postpartum was evaluated by blood chemical analysis. Based on AUCglu changes from Pre21 to Pre10, cows were classified into either the AUC-up group (AUCglu increase, n=5)... |
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Palavras-chave: Dairy cows; Glucose metabolism; Insulin tolerance test; Reproductive performance; Transition period. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4129 |
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| KAWASAKI, Yurie; AOKI, Yuka; MAGATA, Fumie; MIYAMOTO, Akio; KAWASHIMA, Chiho; HOJO, Takuo; OKUDA, Kiyoshi; SHIRASUNA, Koumei; SHIMIZU, Takashi; 宮本, 明夫; 清水, 隆. |
| The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of polymorphonuclear leukocytes... |
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Palavras-chave: Cattle; Immune function; Polymorphism; Reproductive performance; Tumor necrosis factor α (TNF-α). |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3934 |
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| HAYASHI, Ken-Go; MATSUI, Motozumi; ACOSTA, Tomas J.; KIDA, Katsuya; MIYAMOTO, Akio; 松井, 基純; 木田, 克弥; 宮本, 明夫. |
| Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α.... |
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Palavras-chave: Corpus luteum formation; Cow; Follicle aspiration; LH surge. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2982 |
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| ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h... |
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Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979 |
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| HAYASHI, Ken-Go; BERISHA, Bajram; MATSUI, Motozumi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17β (E2) concentration in follicular fluid (FF) as follows: (1) E2<0.5, (2) 0.5<E2<5, (3) 5<E2<20, (4) 20<E2<180 and (5)... |
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Palavras-chave: Follicular development; Angiopoietin-Tie system; Granulosa cell; Cow. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2975 |
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| ISHII, Mitsuo; KOBAYASHI, Syuichi; ACOSTA, Tomas J.; MIKI, Wataru; YAMANOI, Takahiro; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 石井, 三都夫; 松井, 基純; 三宅, 陽一; 宮本, 明夫. |
| The aim of this study was to clarify the relationship between circulating oxytocin (OT) and PGF2α metabolite (PGFM) in mares at the third stage of labor and placental expulsion time in order to investigate a cause of retained placenta of which the incidence increase in a heavy draft mare. Blood was sampled every 5 min from foaling to expulsion of the placenta in 18 heavy draft mares to evaluate circulating OT and PGFM. The relationships between OT and PGFM concentration and recorded placental expulsion times were investigated. The results were as follows (1) The highest level of OT concentration was observed close to foaling in 15 mares. (2) The OT concentrations close to foaling were variable with a large difference from the lowest concentration, 22.1... |
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Palavras-chave: Heavy draft mare; Oxytocin; 15-keto-dihydro prostaglandin F2α (PGFM); Placental expulsion. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3089 |
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| SHIMIZU, Takashi; JAYAWARDANA, Barana C.; TETSUKA, Masafumi; MIYAMOTO, Akio; 清水, 隆; 手塚, 雅文; 宮本, 明夫. |
| Vascular endothelial growth factor (VEGF) isoforms (VEGF 120 and VEGF 164) secreted by granulosa cells are involved in thecal angiogenesis during follicular development in the bovine ovary. However, whether the transcript of the VEGF120 and VEGF164 isoforms differs during follicular development in the ovary is still unknown. We first examined the gene expression of VEGF120, VEGF164, fms-like tyrosine kinase (Flt-1), and fetal liver kinase (Flk-1) in the granulosa cells (GCs) and theca cells (TCs) of pre-selection and post-selection follicles (PRF and POF respectively) from the bovine ovary. Then we examined the effects of FSH and estradiol (E2) on these factors in cultured bovine GCs. Messenger RNA (mRNA) expression was quantified using real-time PCR... |
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Palavras-chave: Estradiol; Fetal liver kinase (Flk-1); Fms-like tyrosine kinase (Flt-1); Follicle-stimulating hormone (FSH); Granulosa cell; Vascular Endothelial Growth factor (VEGF). |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4057 |
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| MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆. |
| We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis. |
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Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043 |
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| MAGATA, Fumie; SHIRASUNA, Koumei; STRÜVE, Klaas; HERZOG, Kathrin; SHIMIZU, Takashi; BOLLWEIN, Heinrich; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫. |
| Persistence of the corpus luteum (CL) in cattle usually occurs during the puerperium and is associated with interference of prostaglandin (PG) F2α release from the uterus. The objective of the present study was to determine for the first time the gene expressions in the persistent CL compared with the CL of pregnancy and cyclic CL. Three types of CL biopsy samples were collected from 32 lactating Holstein cows: (1) CL persisting for 29 to 33 days after the first ovulation postpartum (persistent CL, n=9), (2) CL between days 29 and 33 of early pregnancy (CL of pregnancy, n=8) and (3) CL between days 10 and 13 of the estrous cycle (cyclic CL, n=27). mRNA expression of 2',5'-oligoadenylate synthetase-1 was upregulated only in the CL of pregnancy, confirming... |
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Palavras-chave: Dairy cow; Early pregnancy; Persistent corpus luteum; MRNA expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3938 |
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| HORI, Takuya; YAMSAARD, Thicomeporn; UETA, Yoshiko Yanagimoto; HARAKAWA, Shinji; KANEKO, Etsushi; MIYAMOTO, Akio; XUAN, Xuenan; TOYODA, Yutaka; SUZUKI, Hiroshi; 宮本, 明夫; 玄, 学南; 鈴木, 宏志. |
| It is well-known that there are considerable strain differences in the relative copulation rates between male and superovulated female mice. In particular, the C57BL/6J strain of mice has a lower rate of successful copulation. We examined the effect of exposure to an electric field on sexual behavior in C57BL/6J male mice. When C57BL/6J males were exposed to a 50 Hz, 45 kV/m electric field for 30 min per day for 11 days and placed in a cage with a superovulated female of the same strain, the successful copulation rates of males was significantly improved compared with unexposed males (P<0.05). These results suggest that the exposure of C57BL/6J male mice to an electric field improves their sub-fertility activity in mating with superovulated females. |
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Palavras-chave: Mice; Male; Electric field; Copulation; Superovulation; Sexual behavior. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3021 |
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| SHIMIZU, Takashi; AKIYAMA, Hirotada; ABE, Yasuyuki; SASADA, Hiroshi; SATO, Eimei; MIYAMOTO, Akio; UCHIDA, Takafumi; 清水, 隆; 宮本, 明夫. |
| Protein phosphorylation on certain serine or threonine residues preceding proline (Ser/Thr-Pro) is a pivotal signaling mechanism in diverse cellular processes. Pin1 is a highly conserved enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Although much protein is phosphorylated in the ovary, the role of Pin1 in the ovary is still unknown. The purpose of this study is to investigate the effects of gonadotropins on protein and mRNA expression of Pin1 in mice ovaries. Quantitative PCR analysis showed that the expression of Pin1 mRNA significantly increased in the ovaries of equine chorionic gonadotropin (eCG)-treated mice compared with those of untreated mice (P<0.05). However,... |
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Palavras-chave: E2F; Follicle development; Gonadotropin; Ovary; Pin1. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4056 |
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| MIYAMOTO, Akio; SHIRASUNA, Koumei; HAYASHI, Ken-Go; KAMADA, Daichi; KAWASHIMA, Chiho; KANEKO, Etsushi; ACOSTA, Tomas J.; MATSUI, Motozumi; 宮本, 明夫; 白砂, 孔明; 川島, 千帆; 松井, 基純. |
| Ultrasonography (US) has been applied to the ovary and the uterus of domestic animals from the late 1980s, and established in 1990s as a practical tool for animal production. US made it possible to detect pregnancy at a very early stage and, most importantly, to observe the real-time dynamics of follicular development and hence the discovery of follicular waves. This has greatly contributed to our understanding of ovarian physiology and helped us to develop several "pin-point" protocols for hormonal treatment. While US may not seem to fit preconceived ideas of a "green" technology, it does not contravene environmental priorities, and it is non-invasive ("ethical") and non-hormonal ("clean"). Using the US technology that is now commercially available at a... |
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Palavras-chave: Color Doppler ultrasound; Blood flow; Ovary; Reproduction; Cow. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2983 |
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| SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫. |
| In this study, we examined the existence and structure of areolae and the steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta morphologically and immunohistochemically. Placentas were collected from the 15th, 16th and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were recognized in observations of serial sections. The opening of the areolar cavity... |
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Palavras-chave: Antarctic minke whale; Areola; Balaenoptera bonaerensis; Placenta; Steroidogenesis. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3861 |
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| TANAKA, Jun; ACOSTA, Tomas J.; BERISHA, Bajram; TETSUKA, Masa; MATSUI, Motozumi; KOBAYASHI, Shuichi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Local angiogenesis and angiolysis in the corpus luteum (CL) relate to the luteal function. Recent studies indicate that angiopoietins (ANPT) and their receptors Tie regulate remodeling of microvasculature. We therefore examined 1) the relative changes in the expression of mRNA for ANPT-1, ANPT-2, Tie1 and Tie2 in bovine CL by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) during the estrous cycle and prostaglandin F2α (PGF2α)-induced luteolysis, and 2) the effect of ANPT-2 on progesterone (P4) release from CL at the late stage of the estrous cycle by an in vitro microdialysis system (MDS). The CLs were classified into 4 stages (early: Day 2-5, n=7, mid: Day 8-12, n=15, late: Day 15-17, n=9, regressing: Day >18, n=19). The... |
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Palavras-chave: Angiopoietin; Angiolysis; Luteolysis; Corpus luteum; Cow. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2977 |
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| KOBAYASHI, Shu-ichi; MIYAMOTO, Akio; 宮本, 明夫. |
| Luteal cells utilize lipoproteins (LIPs) for progesterone (P) synthesis. A luteolysin prostaglandin F2α (PGF 2α) has shown to prevent the LIPs utilization for steroidogenesis in bovine midcycle luteal cells. However, a luteolytic injection of PGF2α before Day 5 (Day 0=estrus) can not induce the regression of the corpus luteum (CL). This suggests that the intraluteal mechanism of PGF2 α action on the LIPs utilization in early CL is different from that of the mid luteal stage. Thus, this study aimed to investigate the interaction between PGF2 α and LIPs on the secretion of P and oxytocin (OT) from bovine early CL. An in vitro microdialysis system (MDS) was used as a model. Each bovine early CL (Days 3-4) was implanted with the MDS, and maintained in an organ... |
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Palavras-chave: PGF2α; Lipoproteins; Early CL; Cow. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2972 |
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| TETSUKA, Masafumi; NISHIMOTO, Hiromi; MIYAMOTO, Akio; OKUDA, Kiyoshi; HAMANO, Seizo; 手塚, 雅文; 宮本, 明夫. |
| Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A... |
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Palavras-chave: Bovine; Cortisol; 11β-HSD; Follicle; Glucocorticoid receptor; MRNA. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810 |
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| KAWASHIMA, Chiho; KIDA, Katsuya; MATSUHASHI, Miki; MATSUI, Motozumi; SHIMIZU, Takashi; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 松井, 基純; 清水, 隆; 石井, 三都夫; 三宅, 陽一; 宮本, 明夫; 松長, 延吉. |
| The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the... |
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Palavras-chave: Japanese Black cattle; Metabolic status; Obesity; Reproductive performance; Suckling. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2991 |
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| ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and... |
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Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980 |
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| Registros recuperados: 42 | |
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