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Variáveis que influenciam a produção de celulases e xilanase por espécies de Aspergillus PAB
Sales,Marilia Ribeiro; Moura,Rosemery Batista de; Porto,Tatiana Souza; Macedo,Gorete Ribeiro de; Porto,Ana Lúcia Figueiredo.
O objetivo deste trabalho foi determinar as variáveis que influenciam a produção simultânea de celulases e xilanase por Aspergillus aculeatus URM 4953 e A. phoenicis URM 4924, com uso de bagaço de cana-de-açúcar como substrato. As variáveis avaliadas foram pH inicial, tempo de cultivo, concentração do substrato, agitação, concentração do inóculo, temperatura, tipo de bagaço de cana-de-açúcar e espécie de Aspergillus. Foi utilizado o planejamento fatorial fracionário 2(8-4), com três repetições no ponto central. Todas as variáveis analisadas influenciaram a produção de pelo menos uma das enzimas. As maiores atividades enzimáticas observadas foram: celulases totais, 0,45 UI mL-1; endoglucanase, 0,60 UI mL-1; exoglucanase, 0,17 U mL-1 ; celobiase 6,42 UI...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus aculeatus; Aspergillus phoenicis; Bagaço de cana-de-açúcar; Celobiase; Endoglucanase; Exoglucanase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001100009
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Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis BABT
Pagnoncelli,Maria Giovana Binder; Araújo,Nathália Kelly de; Silva,Nayane Macêdo Portela da; Assis,Cristiane Fernandes de; Rodrigues,Sueli; Macedo,Gorete Ribeiro de.
The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v) of soluble chitosan as carbon source. The enzyme production was strongly repressed by the presence of glucose. The production started as soon as the available sugars finished in the culture medium. The maximum level of chitosanase activity was 500 U.L-1 at 36°C after 36 h incubation. The crude enzyme was optimally active at pH 6.0 and 55°C and in these conditions, the enzyme presented good stability (6 days). The enzyme without purification was used to hydrolyze the chitosan which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosanase; Paenibacillus ehimensis; Chitosanase production; Crude enzymes and TLC.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600023
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Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus BABT
Almeida,Andréa Farias de; Macedo,Gorete Ribeiro de; Chan,Leslie Chee Loong; Pedrini,Márcia Regina da Silva.
In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Baculovirus; In vitro production; Kinetic analysis; SfMNPV.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006
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Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli BJM
Vaz,Michelle Rossana Ferreira; França,Ricardo Luiz Soares de; Andrade,Sirtys Santos Lessa de; Sousa Junior,Francisco Canindé de; Santos,Everaldo Silvino dos; Martins,Daniella Regina Arantes; Macedo,Gorete Ribeiro de.
With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cultivation; Recombinant protein; Visceral leishmaniasis; Escherichia coli.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021
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