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| Magalhães,BSN; Pereira,VLA; Machado,LS; Dias,TS; Balthazar,DA; Barreto,ML; Troccoli,F; Cunha,NC; Nascimento,ER; Almeida,FM; Almosny,NR. |
| ABSTRACT The present study aimed to investigate, by culture and PCR, the occurrence of Mollicutes, Mycoplasma gallisepticum and Mycoplasma synoviae in free-living Muscovy-ducks (Cairina moschata) from the Rio Zoo, RJ, Brazil. Tracheal swabs were obtained from 82 asymptomatic ducks and the samples were submitted to culture of mycoplasmas and PCR for identification of Mollicutes Class, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). Samples were also analyzed directly by PCR, without prior culture, for Mollicutes, MG and MS. Eighteen (18/82) Muscovy-ducks were positive for Mollicutes by culture, all isolates were confirmed as Mollicutes and seven were identified as MG. Of the samples analyzed directly by PCR, without prior culture, 17,1% (14/82)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anseriformes; Free-living; Mollicute Class; Muscovy-ducks; Mycoplasmosis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400315 |
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| Santos,FC dos; Brandão,MDM; Silva,CC da; Machado,LS; Soares,MV; Barreto,ML; Nascimento,ER do; Pereira,VLA. |
| A farm with 3,000 free-range hens between 24 and 65 weeks of age was investigated. These hens were separated in small flocks of 400 to 700 birds, presenting 10 to 23% egg production reduction. Twenty serum samples were collected during the period of drop in egg production and three weeks later for the investigation of Mycoplasma synoviae (MS), M. gallisepticum (MG) and Infectious Bronchitis Virus (IBV) antibodies using ELISA. At the time of the second collection, egg production had resumed to normal levels; however, with 10.23% of the eggs showed eggshell abnormalities limited to the apex. Eggshell strength was significantly different between normal and those with eggshell apex abnormalities, but not other egg-quality parameters. ELISA tests showed that MS... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eggs; Mycoplasm; Egg defects; Egg abnormality; Economic loss. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200015 |
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| Santos,FF; Li,J; Brandão,MDM; Machado,LS; Soares,NM; Nascimento,ER; Khan,MI; Pereira,VLA. |
| ABSTRACT The objective of this study was to identify the species and characterize the genetic relationships among mycoplasma isolates from commercial layer hen flocks using 16S-23S rDNA intergenic spacer region (IGSR) sequencing. Twenty-one isolates were obtained from samples collected from commercial layer flocks in four Brazilian states: São Paulo, Minas Gerais, Rio de Janeiro and Espírito Santo. The isolates were recovered from the São Paulo, Rio de Janeiro and Espírito Santo states. Eleven isolates were originated from tracheal swabs, five from shell gland swabs and five from ovary fragment collection. The 16S-23S rDNA IGSR of isolates were amplified by PCR, and the obtained products were subsequently sequenced. The consensus of each isolate was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma gallinaceum; Mycoplasma pullorum; Mycoplasma gallisepticum; Intergenic spacer region; 16S-23S. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200309 |
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