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| Registros recuperados: 13 | |
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| Oliveira,Tatiana Roselena de; Jaccoud-Filho,David de Souza; Henneberg,Luciana; Michel,Milton Domingos; Demiate,Ivo Mottin; Pinto,Alessandra Teixeira Barbosa; Machinski Junior,Miguel; Barana,Ana Cláudia. |
| This work had as objectives the study of the physical and mechanical characteristics of maize kernels in relation to the contamination by Fusarium sp and by zearalenone in twenty landraces of maize from the southern region of Brazil. From the analyzed samples, 60 % has been considered to have a hard endosperm type and 40 % an intermediary one. A correlation between the physical and mechanical variables was observed as an indication that the higher is the proportion of hard endosperm, more dense will be the kernel and more force for its rupture will be necessary. The level of contamination by Fusarium sp was between 5.5 and 24.75% among the analyzed grains, correlating positively with the flotation index, indicating that the landraces of maize with a softer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Texture of endosperm; Corn landraces; Mycotoxins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700002 |
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| Oliveira,Renata Cabrera de; Bando,Érika; Machinski Junior,Miguel. |
| The aim of this work was to optimize and validate, by intralaboratorial procedures, a method for the determination of patulin in grapes by thin-layer chromatography. The steps of extraction, cleanup, detection and quantification were optimized. For the validation of the method, recovery assays with standard solutions and artificially contaminated samples were carried out. The mean recovery and the variation coefficient were 65.4% and 7.58%, respectively. The optimized conditions were: 50 mL of grape juice, three extraction stages (with 100 mL of ethyl acetate in the first stage and 50 mL in second and third stages), and 100 µL of ethanol to solubilize the extract. The solvent-system used was toluene - ethyl acetate - formic acid (6:3:1), and 0.5% MBTH in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Patulin; Mycotoxins; Grape; Thin-layer chromatography. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200023 |
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| Sekiyama,Beatriz Leiko; Ribeiro,Alessandra Braga; Machinski,Paulo André; Machinski Junior,Miguel. |
| The occurrence of aflatoxins B1 (AFB1), B2 (AFB2 ), G1 (AFG1 ) and G2 (AFG2 ), ochratoxin A (OA) and zearalenone (ZEA) was evaluated in 121 maize-based food samples collected in the commerce of Maringá City, Paraná State, Brazil. The study was carried out between January 2002 and February 2003. Thin-layer chromatographic method was used to determine the mycotoxins. The recovery averages were 106.6%, 109.4%, 106.6%, 109.4%, 101.8% and 101.7% to AFB1, AFB2, AFG1, AFG2, OA and ZEA, respectively. Three samples (2.5%) were positive to AFB1 (8 to 59 µg/kg), two (1.7%) to AFB2 (2.4 µg/kg), one (0.8%) to OA (64 µg/kg) and one (0.8%) to ZEA (448 µg/kg). The greater frequency of positive samples and also the highest concentration of AFB1 were found in popcorn... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycotoxins; Risk; Brazilian products derived from maize. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300016 |
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| Trevisan,Daliah Alves Coelho; Silva,Alex Fiori da; Negri,Melyssa; Abreu Filho,Benício Alves de; Machinski Junior,Miguel; Patussi,Eliana Valéria; Campanerut-Sá,Paula Aline Zanetti; Mikcha,Jane Martha Graton. |
| ABSTRACT The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carvacrol; Biofilm; Antibacterial activity; Salmonella Typhimurium.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100612 |
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| Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel. |
| Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011 |
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| Menotti,Vinicius Stela; Scanferla,Deborah Thais Palma; Oliveira,Kleber Ota de; Fratucci,Gabriela Felber; Alves,Gessé de Souza; Bando,Erika; Nerilo,Samuel Botião; Oliveira,Magda Lúcia Félix de; Machinski Junior,Miguel; Mossini,Simone Aparecida Galerani. |
| Abstract The present study describes a method for simultaneous analysis of cocaine, benzoylecgonine and cocaethylene in urine samples. After solid phase extraction, Gas Chromatography-Mass Spectrometry was used for identification and quantification. The calibration curves were linear at 20 - 3000 ng/mL, r2 0.9997 for benzoylecgonine, 15 - 2000 ng/mL, r2 0.9985 and r2 0.9993 for cocaine and cocaethylene, respectively. Accuracy values: cocaine: 93,5 - 102,1%; benzoylecgonine: 97,5 - 104,8%; cocaethylene: 90,6 - 101,5%. Precision values: cocaine: C.V 5,4 - 14,6%; benzoylecgonine: C.V 7,8 - 12,3%; cocaethylene: C.V 5,9 - 12,3%. Detection and quantification limit values: cocaine and cocaethylene:10 ng/mL and 15 ng/mL, respectivalty; benzoylecgonine:15 ng/mL and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Analytical validation; GC-MS; Cocaine; Human urine. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100627 |
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| Nerilo,Samuel Botião; Martins,Fernanda Andrade; Nerilo,Luciana Botião; Salvadego,Valter Eduardo Cocco; Endo,Renan Yoshio; Rocha,Gustavo Henrique Oliveira; Mossini,Simone Aparecida Galerani; Janeiro,Vanderly; Nishiyama,Paula; Machinski Junior,Miguel. |
| A controlled cross-sectional study of family growers of fruit and vegetables was conducted between October 2009 and October 2010 to characterize the use of pesticides, establish the socio-demographic profile, and analyze cholinesterase activity in small-scale agricultural workers in Southern Brazil. Data was collected for 173 workers and 179 controls. A structured questionnaire was applied collecting socio-demographic information and determining knowledge and work practices in relation to pesticide use. The benchmarks for total cholinesterase (ChEs) and butyrylcholinesterase (BuChE) were obtained from the average enzymatic activity of the occupationally unexposed group (control). The mean age of the exposed population was 40.0 ± 11.2 years. The symptoms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pesticides/use; Pesticides/occupational exposure; Cholinesterase/evaluation/exposure to pesticides; Agricultural workers/exposure to pesticides; Environmental health; Brazil/southern/environmental health. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400783 |
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| Faria,Carla Bertechini; Almeida-Ferreira,Giovana Caputo; Gagliardi,Karina Bertechine; Alves,Tatiane Cristina Albuquerque; Tessmann,Dauri José; Machinski Junior,Miguel; Barbosa-Tessmann,Ione Parra. |
| The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulgur wheat; Fusarium graminearum; PCR; Molecular detection; Food protection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
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| Arroteia,Carla Cristina; Kemmelmeier,Carlos; Machinski Junior,Miguel. |
| A Região Sul do Brasil é grande produtora de maçã, sendo 80% destinada ao consumo in natura. As micotoxinas são metabólitos secundários de fungos presentes na cadeia alimentar como contaminantes, causando diversos efeitos toxicológicos e imunológicos. Considerando que a patulina seja uma micotoxina produzida por Penicillium expansum, principal contaminante da maçã propôs-se investigar a ação de extratos aquoso e oleoso de Nim (Azadirachta indica) em maçã artificialmente contaminada. Foram testados dois tipos de extratos: o extrato aquoso obtido de maceração de folhas de Nim nas concentrações de 5, 10, 20 e 30%, e o extrato oleoso comercial (DalNeem®), obtido de sementes de Nim, nas concentrações de 0,125, 0,25, 0,5, 1, 2 e 5%. Os extratos aquosos não... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azadirachta indica; Maçã; Nim; Patulina; Penicillium expansum. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600002 |
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| Santos,Alini Cristini dos; Souza,Aline Amenencia de; Silva,Milena Veronezi; Nerilo,Samuel Botião; Souza,Alexandra Perdigão Maia de; Bando,Érika; Machinski Junior,Miguel. |
| ABSTRACT: Peanuts are a nutritious food consumed worldwide. Some species of the genus Aspergillus, producers of aflatoxins, colonize peanuts. Human exposure to aflatoxins occurs by ingesting contaminated foods. The objective of this paper was to assess the occurrence and dietary exposure of the population to aflatoxins in peanuts commercially available in Maringa, Brazil, from May 2013 to April 2017. A total of 104 peanut samples were evaluated by thin-layer chromatography and confirmed by derivatization with trifluoroacetic acid. The contamination rate detected was 24.0% in average concentration of 13.4µg/kg. Twenty positive samples (19.2%) exceeded the maximum level permitted in Brazil for the sum of four aflatoxins. Estimated probable daily intake was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Aspergillus flavus; Peanuts; Risk assessment; Public health. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600750 |
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| Caldas,Glória Maria Menezes; Oliveira,Renata Cabrera de; Tessmann,Dauri José; Machinski Junior,Miguel. |
| A podridão ácida é uma patologia das videiras, de alta freqüência nos períodos quentes e chuvosos, em conseqüência do desenvolvimento de fungos e bactérias em seus frutos. Um dos gêneros de fungos responsáveis por esta doença é o Penicillium spp., que poderá levar à produção de micotoxinas, como a patulina. Neste trabalho, foi realizada uma investigação sobre a podridão ácida e a ocorrência de patulina em uvas Itália (Vitis vinifera L. cv. Rubi) cultivadas no município de Marialva, Brasil. Foram avaliados os seguintes tratamentos: (I) testemunha sem sintomas de podridão ácida; (II) amostras com sintomas, sem a remoção das bagas infectadas; (III) amostras com sintomas, com a remoção das bagas infectadas, (IV) amostras com sintomas da doença, com a remoção... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Patulina; Videira; Fitopatologia; Micotoxinas. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000100003 |
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| Registros recuperados: 13 | |
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