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	Provedor de dados 52 (1) 65 (1) 74 (1) Autor Madeira,Humberto Maciel França (3) DallaSanta,Osmar Roberto (1) Gabriel,Jane Eire (1) Gabriel,Jane Eyre (1) Gonçalves,Dicezar (1) Luciano,Fernando Bittencourt (1) Macedo,Renata Ernlund Freitas de (1) Morete,Crisley Aparecida (1) Schühli,Guilherme Schnell e (1) Vicente,Vânia Aparecida (1) Wolupeck,Hanna Lethycia (1) Mais... Palavra-chave Clinical mastitis (1) Comparative genomics (1) Mastitis detection (1) Meat product (1) Molecular markers (1) Multiplex PCR (1) Mycoplasma hyopneumoniae (1) Mycoplasma synoviae (1) Probiotic (1) Staphylococcus aureus (1) Subclinical mastitis (1) Susceptibility (1) Transcription (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2017 (1) 2010 (1) 2007 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil 52 Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida. The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014 Methods for the evaluation of antibiotic resistance in Lactobacillus isolated from fermented sausages 65 Wolupeck,Hanna Lethycia; Morete,Crisley Aparecida; DallaSanta,Osmar Roberto; Luciano,Fernando Bittencourt; Madeira,Humberto Maciel França; Macedo,Renata Ernlund Freitas de. ABSTRACT: The present study aimed to assess the antibiotic resistance in 54 indigenous Lactobacillus plantarum isolated from artisanal fermented sausages. The confirmation of the strain species was performed by multiplex-PCR assay. Antibiotic resistance was assessed by disk diffusion (DD) and Minimum Inhibitory Concentration (MIC) methods. Of 54 L. plantarum, 44 strains were genotypically confirmed as L. plantarum and 3 as Lactobacillus pentosus. The highest resistance rates were to ampicillin and streptomycin. The highest susceptibility rates were shown to tetracycline, chloramphenicol and penicillin G. None of the strains showed multidrug resistance. Resistance rates by DD and MIC were not different (P>0.05) for ampicillin, chloramphenicol,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Meat product; Multiplex PCR; Probiotic; Susceptibility. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800452 Regulation of gene expression in Mycoplasmas: contribution from Mycoplasma hyopneumoniae and Mycoplasma synoviae genome sequences 74 Madeira,Humberto Maciel França; Gabriel,Jane Eyre. This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448) and Mycoplasma synoviae, using a comparative genomics approach to summarize the main features related to transcription and control of gene expression in mycoplasmas. Most of the transcription-related genes present in the three strains are well conserved among mycoplasmas. Some unique aspects of transcription in mycoplasmas and the scarcity of regulatory proteins in mycoplasma genomes are discussed. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma hyopneumoniae; Mycoplasma synoviae; Transcription; Comparative genomics. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200016 Registros recuperados: 3 Primeira ... 1 ... Última

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