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| Magalhães-Matos,Paulo Cesar; Silva,Michele Bahia do Vale; Souza,Paulo Geovani Silva; Lima,Danillo Henrique da Silva; Morais,Rodrigo de; Farias,Diana Maria de; Alvarenga,Laís Cristina Oliveira; Gabriel,Áurea Martins; Oliveira,Wanessa Batista Lima; Góes-Cavalcante,Gustavo; Scofield,Alessandra. |
| ABSTRACT: The objective of this study was to detect helminth eggs and protozoan oocysts in samples of feces from birds of the order Passeriformes in Para State, Brazil. Fecal samples were collected individually from 403 passerine birds seized and kept in captivity in Para State. Samples were processed by the double centrifugation technique in saturated sucrose solution and the coccidial oocyst-positive samples were submitted to sporulation in potassium dichromate 2.0%. Helminth eggs and/or protozoan oocysts were observed in 43.18% (174/403) of the fecal samples examined. Coccidial oocysts were detected in 93.68% (163/174) of the positive samples, whereas helminth eggs were observed in 10.34% (18/174) of the positive samples. Oocyst sporulation occurred in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passerine birds; Endoparasites; Coccidia; Helminths; Brazilian Amazon. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202177 |
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| Magalhães-Matos,Paulo Cesar; Cunha-Santos,Rafaelle; Sousa,Paulo Geovani Silva; Sampaio-Júnior,Francisco Dantas; Barros,Flávia de Nazaré Leite; Mourão,Fábio Rodrigo Paixão; Oliveira,Wanessa Batista Lima; Gabriel,Áurea Martins; Monteiro,Silvia Gonzalez; Góes-Cavalcante,Gustavo; Scofield,Alessandra. |
| ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400663 |
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