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Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco Electron. J. Biotechnol.
Ghaffar Shahriari,Amir; Bagheri,Abdolreza; Bassami,Mohammad Reza; Malekzadeh-Shafaroudi,Saeid; Afsharifar,Alireza; Niazi,Ali.
Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L...
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; ELISA; Recombinant vaccine; Transgenic plants.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006
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