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Molecular characterization of van genes found in vancomycin-resistant Enterococcus spp. isolated from Hospital das Clínicas, FMUSP, São Paulo, Brazil BJID
Caiaffa Filho,H.H.; Almeida,G.D.; Oliveira,G.A.; Sarahyba,L.; Mamizuka,E.M.; Burattini,M.N..
Vancomycin-resistant enterococci strains (VRE) is an important pathogen related with hospital infections in many countries, presenting limited or no therapeutic options for treating serious infections. VRE has presented some different genotypes been VanA and VanB considered to be the most important in hospital environments. In the present study the authors investigated the prevalence of van genes (A, B an C) among clinical isolates of VRE in a five month period at a large tertiary hospital in Sao Paulo, Brazil. The results showed the presence of vanA, but not vanB or vanC in all 43 strains of E. faecalis and five E. faecium studied. The results bring an important issue, due to the possibility of resistance spread of vanA genes, to be monitored and solved...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Enterococci; Vancomycin resistance; Hospital infections.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300001
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Improvement of the indirect hemagglutination test for the detection of antibodies to Streptococcus pyogenes BJMBR
Rubinsky-Elefant,G.; Hoshino-Shimizu,S.; Mamizuka,E.M.; Asciutti,M.M.R..
An indirect hemagglutination test for a seroepidemiological survey of Streptococcus pyogenes infection was standardized. This is an improved modification of the indirect hemagglutination test which utilizes an unstable reagent prepared with fresh blood cells. Two types of bacterial antigens represented by extracellular products and purified streptolysin O were assayed, but only the former antigen gave good results. Pretreatment of the bacterial antigen with 0.15 M NaOH and neutralization to pH 5.5, as well as postfixation of sensitized red cells with 0.1% glutaraldehyde at 56oC for 30 min were found to be essential to give long stability to the reagent in liquid suspension, at least 9 months at 4oC. A total of 564 serum samples with high, moderate and low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus pyogenes; Hemagglutination test; Anti-streptolysin O antibodies.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800008
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Carbapenem-resistant Acinetobacter baumannii outbreak at university hospital BJM
Takagi,E.H.; Lincopan,N.; Cassettari,V.C.; Passadore,L.F.; Mamizuka,E.M.; Martinez,M.B..
Nineteen clonally related imipenem-resistant Acinetobacter baumannii isolates were recovered from eight intensive care unit patients. All isolates harboured blaOXA-51-like β-lactamase genes and showed the absence of 22 kDa fraction in outer membrane porin profile analysis. It suggests a combination of two mechanisms as responsible for carbapenemresistant phenotypes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter; BlaOXA-type genes; Carbapenemases; Nosocomial infection; Brazil.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200024
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