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Bogo,Amauri; Mantle,Peter. |
Analysis of the honeydew from an as yet undescribed, though distinctive, Brazilian Stigmacoccus sp. (near S. asper Hempel) by paper chromatography, Fast atom bombardment (FAB-MS) and Gas chromatography-mass spectrometry (GC-MS) identified fructose and glucose as monosaccharides and sucrose, maltose, trehalulose, trehalose and a hexose-hexitol as disaccharides. Erlose and glucosyl erlose have been identified as the tri- and tetra-saccharides in Stigmacoccus sp. and characterised for the first time in scale insects by modern techniques of linkage analysis. The same erlose oligosaccharides were recognised in honeydew of the common scale insect Coccus hesperidum L., together with the pentamer of this series, maltosyl erlose, therefore recognising that specific... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; FAB-MS; GC-MS; Erlose; Maltose; Metabolic transformation. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000300022 |
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Bogo,Amauri; Mantle,Peter. |
The occurrence of the alditol oligosaccharides in the Claviceps afriana honeydew is partly as a rational expression of the pathogen's selective nutritive metabolism of the sucrose supplied by the host plant. The experiments were carried out in laboratory and when 14C-D-sucrose, 14C-D-fructose or 14C-D-mannitol radiolabelled saccharides were incorporated into: a) sorghum plant infected by C. africana, b) whole and macereted micelia tissue and c) cell-free honeydew of C. africana, it was observed that the glucose moiety of sucrose was not involved in oligosaccharides formation. Glucose was used by the pathogen as nutritional source. Part of the unused fructose moiety was reduced to mannitol by the pathogen's enzymes which was also excreted into honeydew... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biosynthesis; Metabolic pathway; Oligosaccharides; Ergot. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400008 |
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