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Agrobacterium-mediated transformation of white poplar (Populus alba L.): (with 3 figures & 1 table) Phyton
Sánchez,N; Manzanera,JA; Bueno,MA.
A simple method is presented for the genetic transformation of white poplar (Populus alba L.). Root suckers and buds from branches of adult trees were established in vitro. Callus was induced on injured leaves cultured on medium supplemented with 0.45 µM thidiazuron and 0.1 µM a-naphthalene acetic acid. Non-transformed leaves and calli did not survive concentrations higher than 85 µM kanamycin or 18 mM hygromycin. The leaf explants were inoculated with the disarmed Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 2301 strain. Twenty % of the cultured callus explants was kanamycin-resistant two months after the co-inoculation experiment. The rate of adventitious bud induction on resistant calli was 17 %. The analysis of b-glucuronidase expression two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BAP benzil aminopurine TDZ; Thidiazuron IBA; Indole butyric acid NAA; Naphthalene acetic acid KAN; Kanamycin.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572004000100015
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