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| Ma,Huiwen; Wang,Ping; Jin,Dan; Jia,Ting; Mao,Hong; Zhang,Jiandi; Zhao,Shi. |
| Hormones regulate hepatic gene expressions to maintain metabolic homeostasis. Ectonucleotide pyrophosphatase/phosphodiesterase 1 has been thought to interfere with insulin signaling. To determine its potential role in the regulation of metabolism, we analyzed its gene (Enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma HepG2 cells treated with insulin and dexamethasone using northern blot and real-time PCR techniques. Hepatic Enpp1 expression was induced by fasting and reduced by refeeding in the rat liver. In primary rat hepatocytes and HepG2 hepatoma cells, insulin reduced Enpp1 mRNA abundance, whereas dexamethasone induced it. Dexamethasone disrupted the insulin-reduced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ectonucleotide pyrophosphatase/phosphodiesterase 1; Insulin; Dexamethasone; Hepatocytes; Regulation of gene expression. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400609 |
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