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Denadai,Sandra Maria Silveira; Hiane,Priscila Aiko; Marangoni,Sergio; Baldasso,Paulo Aparecido; Miguel,Ana Maria Rauen de Oliveira; Macedo,Maria Lígia Rodrigues. |
Sapucaia (Lecythis pisonis Camb.) raw nuts collected from Brazil were analyzed to determine the proximate composition, amino acid profile of protein fractions, in vitro protein digestibility and antinutritional factors in order to evaluate their potential as a protein alimentary complement. The nuts contained adequate amounts of essential amino acids, fatty acids and minerals. In the present study, no hemagglutinating or inhibitory activities were observed in any of the samples investigated, indicating low or non-detectable levels of proteinase inhibitors or lectins in the samples. In vitro digestibility of in natura and heated nut globulins by mammalian digestive proteinases was carried out using trypsin + chymotrypsin + peptidase, with resulting mean... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein digestibility; Antinutritional factors; Globulins; Proteinases; Lecithys pisonis. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000300018 |
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Haddad Junior,Vidal; Zara,Fernando; Marangoni,Sergio; Toyama,Daniela de Oliveira; Souza,Alex Jardelino Felizardo de; Oliveira,Simone Cristina Buzzo de; Toyama,Marcos Hikari. |
Background: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects ofO. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.Results: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olindias sambaquiensis; Hemolytic; Myonecrosis; Cytotoxicity; Cytolysin; Cnidaria venom. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200316 |
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