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| Fernandes,Andreia V.; Ramos,Márcio V.; Gonçalves,José Francisco C.; Maranhão,Paulo A. C.; Chevreuil,Larissa R.; Souza,Luiz Augusto G.. |
| Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Evolutionary relationships; Plant lectins; Protein-carbohydrate interactions; Swartzia; Tropical Fabaceae. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300008 |
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