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| Marchi, C.; Andersen, L.W.; Panitz, F; Holm, L.-E.; Loeschcke, V.. |
| 454-sequencing of the genome was used to isolate and characterize nuclear 22 microsatellite sequences from the ground beetle Bembidion lampros (Carabidae). A 23 total of 4502 contigs containing di- to penta-nucleotide microsatellite repeats were 24 found (4211 perfect and 289 non-perfect repeats). Primers were developed and tested 25 for 34 microsatellite sequences. Of these 15 were tested on 1140 individual samples 26 amplifying on average 89% of samples and they did not deviate significantly from 27 Hardy-Weinberg and linkage equilibrium. These microsatellite sequences will be useful 28 in studying the effect of agricultural management techniques and various environmental 29 factors on a beneficial agricultural species. |
| Tipo: Journal paper |
Palavras-chave: "Organics" in general. |
| Ano: 2012 |
URL: http://orgprints.org/21072/1/21072.pdf |
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| Ahanchédé, J.E.; Alfaya, F.; Andersen, L.W.; Azam, D.; Bautista, MA.A.M.; Besnard, A.-L.; Bigatti, G.; Bouetard, A.; Coutellec, M.-A.; Ewédjé, E.-E.B.K.; Fuseya, R.; Carcia-Jiménez, R.; Haratian, M.; Hardy, O.J.; Holm, L.-E.; Hoy, C.W.; Koshimizu, E.; Loeschcke, V.; López-Márquez, V.; Machado, C.A.; Machordom, A.; Marchi, C.; Michel, A.P.; Micheneau, C.; Mittapalli, O.; Nagai, T.; Okamoto, N; Pan, Y.; Panitz, F.; Safaie, N.; Sakamoto, T.; Sharifnabi, B.; En-Weitian,; Yu, H.. |
| This article documents the addition of 83 microsatellite marker loci and 96 pairs of single-nucleotide polymorphism (NSP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bembidion lampros, Inimicus japonicus, Lymnaea stagnalis, Panopea abbreviata, Pentadesma butyracea, Sycoscapte rhirticola and Thanatephorus cucumeris (anamorph: Rhizoctonia solani). These loci were cross-tested on the following species: Pentadesma grandiofolia and Pentadesma reyndersii. This article also documents the addition of 96 sequencing primer pairs and 88 allele-specific primers or probes for Plutella zylostella. |
| Tipo: Journal paper |
Palavras-chave: "Organics" in general; Biodiversity and ecosystem services. |
| Ano: 2013 |
URL: http://orgprints.org/24511/1/24511.pdf |
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