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| Marchi,A. M.; Juttel,I. D.; Kawacubo,E. M.; Dalmarco,E. M.; Blatt,S. L.; Cordova,C. M. M.. |
| Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients suspected of having pulmonary tuberculosis, for whom physicians had ordered a baciloscopy test. Baciloscopy was performed using the Ziehl-Neelsen method, and culture was performed by incubation of treated samples in Lowenstein-Jensen's medium at 37ºC for eight weeks. For PCR, DNA was amplified with a specific pair of primers to the M. tuberculosis complex, with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Baciloscopy; Culture; PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400003 |
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