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| Marino,Polyana Carolina; Bizarro-Silva,Camila; Búfalo,Isabela; Rosa,Camila Oliveira; Gonçalves,Gustavo Romero; Lisboa,Livia Aires; Seneda,Marcelo Marcondes. |
| Abstract This study aimed to evaluate different concentrations of growth and differentiation factor-9 (GDF-9) on the development and maintenance of equine preantral follicle morphology during short-term in vitro culture. Ovaries (n=5) from five mares were collected from a local slaughterhouse and transported to the laboratory, where nine fragments (5x5x1mm) were procured from each ovary. One fragment from each was immediately fixed and submitted for histological analysis (control group; D0). The other eight fragments were cultured in situ for two (D2) or six (D6) days in MEM+ or MEM+ supplemented with GDF-9 at different concentrations (i.e., 50, 100 and 200 ng/mL the GDF-9). After culturing with different concentrations of GDF-9 for 2 or 6 days, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Assisted Reproduction; GDF-9; Mare. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100515 |
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