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A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR Biocell
Cardozo,G. P.; Santos,E. V.; Fachin,A. L.; França,S. C.; Marins,M..
E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Ehrlichia canis; Glass bead; Real-time PCR.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100005
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Cell migration in the postnatal subventricular zone BJMBR
Menezes,J.R.L.; Marins,M.; Alves,J.A.J.; Fróes,M.M.; Hedin-Pereira,C..
New neurons are constantly added to the olfactory bulb of rodents from birth to adulthood. This accretion is not only dependent on sustained neurogenesis, but also on the migration of neuroblasts and immature neurons from the cortical and striatal subventricular zone (SVZ) to the olfactory bulb. Migration along this long tangential pathway, known as the rostral migratory stream (RMS), is in many ways opposite to the classical radial migration of immature neurons: it is faster, spans a longer distance, does not require radial glial guidance, and is not limited to postmitotic neurons. In recent years many molecules have been found to be expressed specifically in this pathway and to directly affect this migration. Soluble factors with inhibitory, attractive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Postnatal neurogenesis; Chain migration; Rostral migratory stream; Astrocytes; Gap junctions; Radial glia.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200002
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Analysis of the 16S rRNA gene of Anaplasma platys detected in dogs from Brazil BJM
Cardozo,G.P.; Oliveira,L.P.; Zissou,V.G.; Donini,I.A.N.; Roberto,P.G.; Marins,M..
Comparison of the partial DNA sequence of the 16S rRNA gene of Anaplasma platys detected in dogs from Ribeirão Preto, Brazil, to sequences of other strains previously deposited in GenBank showed that there are at least three A. platys strains circulating in South America.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaplasma platys; 16S rRNA gene; PCR.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300017
Registros recuperados: 3
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