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A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks BJM
Fraga,Aline Padilha; Vargas,Tatiana de; Ikuta,Nilo; Fonseca,André Salvador Kazantzi; Celmer,Álvaro José; Marques,Edmundo Kanan; Lunge,Vagner Ricardo.
Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneous detection of MG and MS by multiplex real time polymerase chain reaction (PCR). The complete assay (Multiplex MGMS) was designed with primers and probes specific for each pathogen and developed to be carried out in a single tube reaction. Vaccines, MG and MS isolates and DNA from other Mycoplasma species were used for the development and validation of the method. Further, 78 pooled clinical samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time PCR; Mycoplasma gallisepticum; Mycoplasma synoviae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200028
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