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Expression of the antibody 14D9 in Nicotiana tabacum hairy roots Electron. J. Biotechnol.
Martínez,Carolina; Petruccelli,Silvana; Giulietti,Ana María; Alvarez,María Alejandra.
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
Tipo: Journal article Palavras-chave: Catalytic antibodies; In vitro cultures; Molecular farming; Recombinant antibodies; Tobacco.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
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Effects on fruit production and quality of different dormant pruning intensities in 'Bing'/'Gisela®6' sweet cherries (Prunus avium) in Central Chile Ciencia e Investigación Agraria
von Bennewitz,Eduardo; Fredes,Claudio; Losak,Tomas; Martínez,Carolina; Hlusek,Jaroslav.
Fruit size is a very important quality attribute in cherry trees. Appropriate canopy and crop load management are, therefore, required to obtain an adequate balance between the yield and fruit size. A study was carried out during the 2007-2008 season in the Maule Region of Chile to evaluate the effect of increasing levels of dormant pruning (Control, no removal; Soft pruning, 15% removal; Moderate pruning, 30% removal and Intense pruning, removal of 50% of the fruiting wood) on the fruit yield, quality (size, fruit weight, soluble solids and fruit firmness) and crop value of sweet cherries (Prunus avium L.) cv. 'Bing' on the dwarfing rootstock, 'Gisela 6'. Treatments at the 30 and 50% removal intensities had a strong effect on yield reduction (36.1% and...
Tipo: Journal article Palavras-chave: Crop load regulation; Dormant pruning; Fruit yield and quality; Prunus avium.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300003
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