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YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana Genet. Mol. Biol.
Munguía-Rodríguez,Aarón Giovanni; López-Bucio,Jesús Salvador; Ruiz-Herrera,León Francisco; Ortiz-Castro,Randy; Guevara-García,Ángel Arturo; Marsch-Martínez,Nayelli; Carreón-Abud,Yazmín; López-Bucio,José; Martínez-Trujillo,Miguel.
Abstract Auxin regulates a plethora of events during plant growth and development, acting in concert with other phytohormones. YUCCA genes encode flavin monooxygenases that function in tryptophan-dependent auxin biosynthesis. To understand the contribution of the YUCCA4 (YUC4) gene on auxin homeostasis, plant growth and interaction with abscisic acid (ABA) signaling, 35S::YUC4 seedlings were generated, which showed elongated hypocotyls with hyponastic leaves and changes in root system architecture that correlate with enhanced auxin responsive gene expression. Differential expression of PIN1, 2, 3 and 7 auxin transporters was detected in roots of YUC4 overexpressing seedlings compared to the wild-type: PIN1 was down-regulated whereas PIN2, PIN3 and PIN7...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabidopsis; Auxin; Abscisic acid; YUCCA4; Root growth; Germination.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100302
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Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins Genet. Mol. Biol.
Solís-Guzmán,María Gloria; Argüello-Astorga,Gerardo; López-Bucio,José; Ruiz-Herrera,León Francisco; López-Meza,Joel; Sánchez-Calderón,Lenin; Carreón-Abud,Yazmín; Martínez-Trujillo,Miguel.
Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spen; Arabidopsis; Vascular bundle; Expression.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400643
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Determinación de la DL50 Y GR 50 con rayos gamma (60Co) en protocormos de Laelia autumnalis in vitro Agrociencia
Hernández-Muñoz,Selene; Pedraza-Santos,M. Elena; López,P. Antonio; Cruz-Torres,Eulogio De La; Fernández-Pavía,S. Patricia; Martínez-Palacios,Alejandro; Martínez-Trujillo,Miguel.
Resumen Laelia autumnalis es una orquídea con valor ornamental alto en México, pero su comercialización se restringe porque las variedades con calidad comercial no existen. La mutagénesis con radiación es un método efectivo para generar variedades y depende de la radiosensibilidad de los tejidos. El objetivo del estudio fue determinar las dosis letal y reductiva media a los rayos gamma en protocormos de L. autumnalis tratados con 0, 5, 10, 15, 20, 25, 30, 35, 40, 45 y 50 Gy. El diseño experimental fue completamente al azar, con tres repeticiones y 500 a 700 protocormos como unidad experimental. A los 45 d después de la irradiación (ddi) se cuantificaron los porcentajes de supervivencia, protocormos fotosintéticos, protocormos con promeristemos, con hojas,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laelia autumnalis; Mejoramiento genético; Mutagénesis; Radiación ionizante; Orquídeas.
Ano: 2017 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952017000500507
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