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Unexpected high abyssal ophiuroid diversity in polymetallic nodule fields of the Northeast Pacific Ocean, and implications for conservation ArchiMer
Christodoulou, Magdalini; O'Hara, Timothy; Hugall, Andrew; Khodami, Sahar; Rodrigues, Clara F.; Hilario, Ana; Vink, Annemiek; Martinez Arbizu, Pedro.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00595/70720/68952.pdf
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A reverse taxonomic approach to assess macrofaunal distribution patterns in abyssal Pacific polymetallic nodule fields ArchiMer
Janssen, Annika; Kaiser, Stefanie; Meissner, Karin; Brenke, Nils; Menot, Lenaick; Martinez Arbizu, Pedro.
Heightened interest in the exploitation of deep seafloor minerals is raising questions on the consequences for the resident fauna. Assessing species ranges and determination of processes underlying current species distributions are prerequisites to conservation planning and predicting faunal responses to changing environmental conditions. The abyssal central Pacific nodule belt, located between the Clarion and Clipperton Fracture Zones (CCZ), is an area prospected for mining of polymetallic nodules. We examined variations in genetic diversity and broad-scale connectivity of isopods and polychaetes across the CCZ. Faunal assemblages were studied from two mining claims (the eastern German and French license areas) located 1300 km apart and influenced by...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00255/36595/35158.pdf
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Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes ArchiMer
Guenther, Babett; Knebelsberger, Thomas; Neumann, Hermann; Laakmann, Silke; Martinez Arbizu, Pedro.
We establish the new approach of environmental DNA (eDNA) analyses for the North Sea. Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase subunit I (COI) gene for analyzing species composition and the nuclear hypervariable region V8 of 18S rDNA for analyzing supraspecific biodiversity. A new minibarcode primer (124 bp) was created on the basis of a metazoan COI barcode library with 506 species and tested in silico, in vitro, and in situ. We applied high throughput sequencing to filtrates of 23 near-bottom water samples taken at three seasons from 14 stations. The set of COI primers allowed amplification of mitochondrial minibarcodes for diverse metazoan phyla and the differentiation at the species level for more than 99%...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00462/57351/59444.pdf
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