|
|
|
|
| |
|
|
| Silva,Carolina Gonzales da; Martins,Carlos Frederico; Cardoso,Tereza Cristina; Cunha,Elisa Ribeiro da; Bessler,Heidi Christina; McManus,Concepta Margaret; Pivato,Ivo; Báo,Sônia Nair. |
| ABSTRACT: Wharton's jelly is a source of mesenchymal stem cells (MSCs) that had not yet been tested for bovine embryo production by nuclear transfer (NT). Thus, the objective of this study was to isolate, characterize and test MSCs derived from Wharton's jelly for embryo and pregnancy production by NT in cattle. The umbilical cord was collected during calving and cells derived from Wharton's jelly (WJCs) were isolated by explant and cultured in Dulbecco's Modified Eagle Medium. Skin Fibroblasts (FB) were isolated after 6 months of life. Morphological analysis was performed by bright field and scanning electron microscopy (SEM) during cell culture. Phenotypic and genotypic characterization by flow cytometry, immunocytochemistry, RT-PCR and differentiation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cloning; Mesenchymal stem cell; Umbilical cord. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001830 |
| |
|
|
| Costa,Priscila de Melo; Martins,Carlos Frederico; Franco,Vanessa de Oliveira; Rezende,Luiz Osvaldo Fonseca; Sereno,José Robson Bezerra; Campos,Haroldo da Costa Ferreira. |
| O objetivo deste estudo foi avaliar as características morfológicas e funcionais dos espermatozóides bovinos recuperados de epidídimos resfriados por longos períodos e posteriormente criopreservados. Testículos bovinos foram coletados no abatedouro, transportados ao laboratório e armazenados a 5°C por 0, 24, 48 e 72 horas (n=10 para cada tratamento). Os espermatozóides foram extraídos de cada epidídimo, avaliados e diluídos em meio tris-gema-glicerol a 7% e criopreservados em nitrogênio líquido. As características morfológicas e funcionais dos espermatozóides foram avaliadas in vitro por análise microscópica e in vivo, por meio de inseminação artificial. Foram observadas alterações morfológicas características da imaturidade dos espermatozóides e redução... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animais mortos; Biotecnologia; Germoplasma. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000500022 |
| |
|
|
| Ferra,Janine de Campos; Cieslak,Silvia; Sartori Filho,Roberto; McManus,Connie; Martins,Carlos Frederico; Sereno,José Robson Bezerra. |
| The objective of this study was to identify and to correlate parameters that characterize puberty and estimate the age and weight of Suffolk ewe lambs in Mato Grosso do Sul. This study was carried out with twenty-two Suffolk ewe lambs for 256 days. Blood samples were collected from the jugular vein and centrifugated and the plasma were stored at -20°C until analysis of progesterone (P4) concentration by radioimmunoassay (RIA). The morphometric measurements included: head length and width, body length, elbow height, chest perimeter, back-sternal diameter, croup length, width and height, cannon bone perimeter and shoulder and hip width, using a tape. The animals became pubertal when the plasma progesterone concentration was >1.0 ng/mL, at 34.1 ± 5.2 kg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chest perimeter; Progesterone; Radioimmnunoassay; Sheep. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000100018 |
| |
|
|
|
