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Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays BJM
Martins,Flaviano S.; Miranda,Ieso C.; Rosa,Carlos A.; Nicoli,Jacques R.; Neves,Maria J..
Probiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of the host when ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed by in vitro assays simulating gastrointestinal environment to determine the ability to survive in the digestive tract. In the present study, the possibility of extrapolation of data obtained in in vitro assays to in vivo conditions was studied using five Saccharomyces cerevisiae strains isolated from Brazilian Atlantic rain forest. Trehalose contents and survival after exposure to a combination of physiological stresses generally found in the gastrointestinal tract of humans were determined for the five yeasts and compared to the behavior...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotic; Saccharomyces boulardii; Saccharomyces cerevisiae; Stress; Trehalose.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100012
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