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| Sanson,Ananda Lima; Silva,Suéllen Cristina Rennó; Martins,Matheus Coutinho Gonçalves; Giusti-Paiva,Alexandre; Maia,Patrícia Penido; Martins,Isarita. |
| A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL-1, for doxorubicin and 1 to 100 µg mL-1, for the other compounds. The relative standard deviations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antineoplastic drugs/simultaneous determination in plasma; Liquid-liquid extraction; High-performance liquid chromatography/diode array detector. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200017 |
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| Machado,Simone Caetani; Landin-Silva,Mariane; Maia,Patrícia Penido; Rath,Susanne; Martins,Isarita. |
| The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 µm) at 40ºC, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min-1, increased by 1.2 mL min-1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 µg kg-1, precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QuEChERS; Sulphonamides determination; Veterinary drugs residues; Chicken breast; HPLC-DAD. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100017 |
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| Alcântara,Adélia Maria Pimenta de Pádua; Vitor,Ricardo Vilela; Vieira,Elisabeth Pizzamiglio; Martins,Isarita. |
| The aim of this study was to develop a method for simultaneous detection of antineoplastic drugs on gloves since, in occupational exposure, the main contamination route is through dermal contact, which may occur via prolonged contact with contaminated surfaces or materials. The assay was performed by liquid chromatography using the following conditions for the detection of 5-fluorouracil (5-FU), methotrexate (MTX) and paclitaxel (TAX): diode array detection and UV quantification at 195 nm for TAX, at 265 nm for 5-FU and at 302 nm for MTX; ODS column (250 x 4 mm, 5 μm) with a similar guard column; mobile phase consisted of water (pH 4)-methanol-acetonitrile (35:15:50, v/v/v) with a flow rate of 1 mL min-1. The method presented a linear range from 0.25 to 20... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antineoplastic drugs/detection; Gloves/toxicologial analysis; High Performance Liquid Chromatography/toxicologial analysis; Occupational exposure. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400015 |
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| Kujbida,Paula; Maia,Patrícia Penido; Araújo,Ariadne Naama de; Mendes,Leonardo Daniel; Oliveira,Mariana Lepri de; Silva-Rocha,Walicyranison Plinio; Brito,George Queiroz de; Chaves,Guilherme Maranhão; Martins,Isarita. |
| In the present study, the occurrence of fungi and aflatoxins (AFs) in peanut and cashew nut samples was investigated. Mycological analysis revealed the presence of fungi in 58.8% of samples, and assessment of AFs by chromatographic methods revealed that 52.9% were contaminated by AFs. AFB1 was the principal component in all AF-contaminated samples, with a mean level of 14.0, and 1.08 µg/kg in peanut and cashew nut, respectively. Eleven samples (32.4%) exceeded the total AF maximum level (4 μg/kg) and 8 samples (23.5%) exceeded the AFB1 (2 μg/kg) established by the European Commission. Our findings suggest that the incidence of AFs emphasizes the need for regular monitoring and a more stringent food safety system to control AFs at the lowest possible levels... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxin; Peanut; Cashew nut; Fungi; Risk assessment. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100502 |
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| Malafatti,Lusiane; Maia,Patrícia Penido; Martins,Matheus Coutinho Gonçalves; Siqueira,Maria Elisa Pereira Bastos de; Martins,Isarita. |
| Nicotine is a major addictive compound in cigarettes and is rapidly and extensively metabolized to several metabolites in humans, including urinary cotinine, considered a biomarker due to its high concentration compared to other metabolites. The aim of this study was to develop a single method for determination of urinary cotinine, in active and passive smokers, by gas chromatography with a nitrogen phosphorus detector (GC-NPD). Urine (5.0 mL) was extracted with 1.0 mL of sodium hydroxide 5 mol L-1, 5.0 mL of chloroform, and lidocaine used as the internal standard. Injection volume was 1 μL in GC-NPD. Limit of quantification was 10 ng mL-1. Linearity was evaluated in the ranges 10-1000 ng mL-1 and 500-6000 ng mL-1, with determination coefficients of 0.9986... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gas chromatography/with nitrogen phosphorus detector; Urinary cotinine/determination; Urine/toxicological analysis; Smokers/Toxicology. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400019 |
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| Santos,Mariane Gonçalves; Vitor,Ricardo Vilela; Nakamura,Maurício Gustavo; Morelini,Luana de Souza; Ferreira,Rafaela Scalco; Paiva,Alexandre Giusti; Azevedo,Luciana; Marques,Vanessa Bergamin Boralli; Martins,Isarita; Figueiredo,Eduardo Costa. |
| Organophosphates (OPs) are widely used as pesticides, and its urinary metabolites as well as the blood cholinesterases (ChEs) activity have been reported as possible biomarkers for the assessment of this pesticide exposure. Moreover, the OPs can induce mutagenesis, and the bone marrow micronucleus test is an efficient way to assess this chromosomal damage. This paper reports a study carried out to verify the correlation among the disulfoton exposure, blood ChEs activity, urinary diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), as well as micronucleated polychromatic erythrocytes (MNPCEs) frequency. Four groups of rats (n=12) were exposed to disulfoton at 0, 2.8, 4.7, and 6.6 mg kg-1 body weight. The blood ChEs activity, urinary DETP and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pesticides/exposure study; Organophosphate/exposure study; Disulfoton/exposure; Dialkyl phosphate/urinary excretion; Blood cholinesterase; Micronucleated polychromatic erythrocytes/frequency. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100016 |
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