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| Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela. |
| ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600455 |
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| Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela. |
| ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of 100... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500451 |
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