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| Bastos,Carla Resende; Mathias,Luis Antonio; Jusi,Márcia Mariza Gomes; Santos,Renata Ferreira dos; Silva,Glaucenyra Cecília Pinheiro da; André,Marcos Rogério; Machado,Rosangela Zacarias; Bürger,Karina Paes. |
| Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564 |
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| Silva,Felipe Jorge da; Silva,Glaucenyra Cecília Pinheiro; Loffler,Sylvia Grune; Brihuega,Bibiana; Samartino,Luis Ernesto; Alarcon,Miguel Frederico Fernandez; Santos,Carlos Eduardo Pereira dos; Mathias,Luis Antonio. |
| The aim of this study was to describe the isolation of a pathogenic strain of Leptospira interrogans from the urine sample of a male human living in the rural area of the County of Cruz Alta, Rio Grande do Sul. An aliquot of each urine sample was sown in a Fletcher and Ellinghausen - McCullough - Johnson - Harris (EMJH) media. Samples in which there was growth of spirochetes were sent to the Leptospirosis Laboratory of the Institute of Pathobiology in the National Institute of Agricultural Technology, Buenos Aires, Argentina and were typified by the Multiple Locus of Variable Number Tandem Repeat technique (MLVA). Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 was isolated, and this is a very important finding that serves as a warning to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human leptospirosis; Isolation; MLVA; Farm; Brazil. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100047 |
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| Mathias,Luis Antonio; Corbellini,Luis Gustavo; Maia,Lúcia; Nascimento,Kelly Fagundes; Paulin,Lília Márcia Silva; Samartino,Luis Ernesto; Serqueira,Marco Antonio; Soares Filho,Paulo Martins; Souza,Marcília Maria Alves de. |
| Esta investigação teve por objetivo validar o teste de polarização fluorescente (TPF) para o diagnóstico sorológico da brucelose bovina, determinando a sensibilidade (SE) e a especificidade relativas (SP) e verificando a reprodutibilidade do teste em quatro laboratórios no Brasil. Foram selecionadas 1.389 amostras de soro sanguíneo, as quais foram inicialmente submetidas aos testes do antígeno acidificado tamponado (AAT) e mercaptoetanol (2-ME). As mesmas amostras foram submetidas à reação de fixação de complemento (RFC) e ao TPF. Para a avaliação do TPF, foi adotada a combinação dos resultados do AAT, da RFC e do 2-ME, utilizados como população de referência (padrão-ouro). Para a determinação do ponto de corte do TPF que proporciona a melhor combinação de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucelose bovina; Diagnóstico sorológico; Teste de polarização fluorescente. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000013 |
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