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| Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.. |
| Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into... |
| Tipo: Text |
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf |
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| Mathieu, M; Leflaive, J; Ten Hage, L; De Wit, R; Buffan Dubau, E. |
| The aim of the present study was to examine the effects of biofilm-dwelling nematode activity on the oxygen turnover of cultured diatom biofilms. Oxygen microprofiles were simultaneously measured using the microelectrode technique in biofilms incubated with and without nematodes during short-term (24 h) and medium-term (6 d) experiments. During 24 h nycthemeral incubations, maximum oxygen concentrations and oxygen peak depths of microprofiles, as well as net oxygen production rates, were significantly higher in the diatom biofilms with nematodes. These effects might be dependent on the densities of nematodes. In the present study, significant effects were observed at nematode densities exceeding 100 ind. cm(-2), but not at a density <50 ind. cm(-2).... |
| Tipo: Text |
Palavras-chave: Bioturbation; Grazing; Net production rate; Oxygen community metabolism; Microelectrode; Oxygen microprofile; Nematodes; Diatom biofilms. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3607.pdf |
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