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Quantitative real-time PCR for the clinical detection of Helicobacter pylori Genet. Mol. Biol.
Ribeiro,Marcelo Lima; Ecclissato,Christina Cunha; Mattos,Ricardo Gabriel; Mendonca,Sergio; Pedrazzoli Jr.,José.
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnostic methods; Helicobacter pylori; Real-time PCR.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022
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