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Cloning of endothelin-1 (ET-1) from European sea bass (Dicentrarchus labrax) and its gene expression analysis in larvae with retinoic acid-induced malformations ArchiMer
Geay, Florian; Darias, Maria J.; Santigosa, E.; Desbruyeres, Elisabeth; Quazuguel, Patrick; Zambonino, Jose-luis; Cahu, Chantal; Mazurais, David.
It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain...
Tipo: Text Palavras-chave: European sea bass; Retinoic acid; Morphogenesis; Gene expression; Endothelin 1.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6172.pdf
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Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry ArchiMer
Callet, Therese; Dupont-nivet, Mathilde; Cluzeaud, Marianne; Jaffrezic, Florence; Laloe, Denis; Kerneis, Thierry; Labbe, Laurent; Quillet, Edwige; Geurden, Inge; Mazurais, David; Skiba-cassy, Sandrine; Medale, Francoise.
o meet the growing demand of fish feed for aquaculture, an increasing proportion of marine ingredients are being replaced by blends of plant products. However, the total replacement of marine ingredients in salmonid diets impairs fish performance. This is particularly true during the early fry stage and this stage is therefore considered of particular importance. In rainbow trout (RBT), the existence of a genetic variability to survive and grow with plant-based diets devoid of marine ingredients has now been proved, but the mechanisms behind are little studied especially at early stage. To investigate these, we analysed the whole transcriptome of three isogenic lines of RBT fry, which have similar growth when fed a marine resources-based diet (M diet) but...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00452/56371/57969.pdf
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An early-life hypoxia event has a long-term impact on protein digestion and growth in European sea bass juvenile. ArchiMer
Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy.
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined...
Tipo: Text Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf
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Moderate hypoxia but not warming conditions at larval stage induces adverse carry-over effects on hypoxia tolerance of European sea bass ( Dicentrarchus labrax ) juveniles ArchiMer
Cadiz, Laura; Ernande, Bruno; Quazuguel, Patrick; Servili, Arianna; Zambonino-infante, Jose-luis; Mazurais, David.
Environmental conditions, to which organisms are exposed during all their life, may cause possible adaptive responses with consequences in their subsequent life-history trajectory. The objective of this study was to investigate whether ecologically relevant combinations of hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), occurring during the larval period of European sea bass larvae (Dicentrarchus labrax), could have long-lasting impacts on the physiology of resulting juveniles. Hypoxic challenge tests were performed over one year to give an integrative evaluation of physiological performance. We revealed that juvenile performance was negatively impacted by hypoxia but not by the thermal conditions experienced at larval stage. This...
Tipo: Text Palavras-chave: Hypoxia; Temperature; Developmental plasticity; Carry-over effects; Opercular malformation; European sea bass.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00434/54606/55988.pdf
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Hypoxic episode during the larval period has long-term effects on European sea bass juveniles (Dicentrarchus labrax) ArchiMer
Vanderplancke, Gwenaelle; Claireaux, Guy; Quazuguel, Patrick; Madec, Lauriane; Ferraresso, Serena; Severe, Armelle; Zambonino-infante, Jose-luis; Mazurais, David.
Hypoxia episodes have been generally studied over the last decade for their lethal consequences in coastal areas, which are nurseries for several fish species. The possible long-lasting effects of non-lethal hypoxia exposures at larval stage are, however, poorly documented. We investigated the long-lasting phenotypic impact of an experimental exposure to moderate hypoxia (40 % air saturation) between 30 and 38 days post-hatching in European sea bass (Dicentrarchus labrax). At the juvenile stage, our data indicate that this early hypoxia exposure impacted on juvenile growth rate but not on hypoxia tolerance. Growth depression was associated with a regulation of metabolism as suggested by a decrease in blood glycaemia, a decreased hepatic expression of the...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36517/36676.pdf
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Long-term exposure to near-future ocean acidification does not affect the expression of neurogenesis- and synaptic transmission-related genes in the olfactory bulb of European sea bass (Dicentrarchus labrax) ArchiMer
Mazurais, David; Servili, Arianna; Le Bayon, Nicolas; Gislard, Sebastien; Madec, Lauriane; Zambonino-infante, Jose-luis.
The decrease in ocean pH that results from the increased concentration of dissolved carbon dioxide (CO2) is likely to influence many physiological functions in organisms. It has been shown in different fish species that ocean acidification (OA) mainly affects sensory systems, including olfaction. Impairment of olfactory function may be due to a dysfunction of the GABAergic system and to an alteration of neuronal plasticity in the whole brain and particularly in olfactory bulbs. Recent studies revealed that OA-driven effects on sensory systems are partly mediated by the regulation of the expression of genes involved in neurotransmission and neuronal development. However, these studies were performed in fish exposed to acidified waters for short periods, of...
Tipo: Text Palavras-chave: Ocean acidification; Long-term exposure; Gene expression; Olfactory bulbs.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71786/70315.pdf
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Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota ArchiMer
Lamari, Faouzi; Castex, Mathieu; Larcher, Thibaut; Ledevin, Mireille; Mazurais, David; Bakhrouf, Amina; Gatesoupe, Joel.
Probiotics may have many effects on health and development of fish larvae. One of the most promising is related to spinal conformation, though the mode of action is not clearly understood. The present study attempted to investigate the effects of two strains of lactic acid bacteria on associated microbiota, histological development and gene expression. Sea bass larvae were fed since 5 dph (day post hatch) with either a standard control diet (Diet C), or the same diet supplemented with Pediococcus acidilactici MA18/5M (Diet P), or with an autochthonous strain of Lactobacillus casei (X2; Diet L). The two lactic acid bacteria were incorporated in the diets at the levels of 106 and 107 CFU (colony forming units) g-1 in two consecutive experiments,...
Tipo: Text Palavras-chave: Fish larvae; Probiotics; Microbiota; Histopathology; Gene expression; DGGE.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00108/21966/19673.pdf
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Animal board invited review: OneARK: Strengthening the links between animal production science and animal ecology ArchiMer
Destoumieux-garzón, Delphine; Bonnet, P.; Teplitsky, C.; Criscuolo, F.; Henry, P.-y.; Mazurais, David; Prunet, P.; Salvat, Gilles; Usseglio-polatera, P.; Verrier, E.; Friggens, N.c..
Wild and farmed animals are key elements of natural and managed ecosystems that deliver functions such as pollination, pest control and nutrient cycling within the broader roles they play in contributing to biodiversity and to every category of ecosystem services. They are subjected to global changes with a profound impact on the natural range and viability of animal species, the emergence and spatial distribution of pathogens, land use, ecosystem services and farming sustainability. We urgently need to improve our understanding of how animal populations can respond adaptively and therefore sustainably to these new selective pressures. In this context, we explored the common points between animal production science and animal ecology to identify promising...
Tipo: Text Palavras-chave: Adaptation; Agroecosystem; Livestock sustainability; Resilience; Zoonotic disease.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00663/77509/79273.pdf
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Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth ArchiMer
Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier.
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel...
Tipo: Text Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf
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Metabolic response to hypoxia in European sea bass (Dicentrarchus labrax) displays developmental plasticity ArchiMer
Cadiz, Laura; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Madec, Lauriane; Le Delliou, Herve; Mazurais, David.
Several physiological functions in fish are shaped by environmental stimuli received during early life. In particular, early-life hypoxia has been reported to have long-lasting effects on fish metabolism, with potential consequences for fish life history traits. In the present study, we examine whether the synergistic stressors hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), encountered during early life, could condition later metabolic response in European sea bass (Dicentrarchus labrax) juveniles. Growth rate and metabolic parameters related to carbohydrate and lipid metabolism in the liver were investigated at the juvenile stage under normoxic and chronic hypoxic conditions. Juvenile growth rates were significantly lower...
Tipo: Text Palavras-chave: Hypoxia; Developmental plasticity; Metabolism; European sea bass.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00404/51536/52126.pdf
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De novo assembly, characterization and functional annotation of Senegalese sole (Solea senegalensis) and common sole (Solea solea) transcriptomes: integration in a database and design of a microarray ArchiMer
Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel.
Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from...
Tipo: Text Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf
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The development of contemporary European sea bass larvae (Dicentrarchus labrax) is not affected by projected ocean acidification scenarios ArchiMer
Crespel, Amelie; Zambonino-infante, Jose-luis; Mazurais, David; Koumoundouros, George; Fragkoulis, Stefanos; Quazuguel, Patrick; Huelvan, Christine; Madec, Lauriane; Servili, Arianna; Claireaux, Guy.
Ocean acidification is a recognized consequence of anthropogenic carbon dioxide (CO2) emission in the atmosphere. Despite its threat to marine ecosystems, little is presently known about the capacity for fish to respond efficiently to this acidification. In adult fish, acid-base regulatory capacities are believed to be relatively competent to respond to hypercapnic conditions. However, fish in early life stage could be particularly sensitive to environmental factors as organs and important physiological functions become progressively operational during this period. In this study, the response of European sea bass (Dicentrarchus labrax) larvae reared under three ocean acidification scenarios, i.e., control (present condition, P-CO2 = 590 mu atm, pH total =...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00393/50448/51181.pdf
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Probiotic treatment of live food organisms for Atlantic bluefin tuna larvae: microbiological & immunological criteria ArchiMer
Coves, Denis; De Vogue, Benoist; Desbruyeres, Elisabeth; Dhormes, Benoit; Fievet, Julie; Huelvan, Christine; Lallement, Stephane; Le Gall, Marie-madeleine; Ruelle, Francois; Vidal, Marie-odile; Castex, M.; Mazurais, David; Cahu, Chantal; Gatesoupe, François-joel.
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16189/13702.pdf
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Constraints and Priorities for Conducting Experimental Exposures of Marine Organisms to Microplastics ArchiMer
Paul-pont, Ika; Tallec, Kevin; Gonzalez-fernandez, Carmen; Lambert, Christophe; Vincent, Dorothee; Mazurais, David; Zambonino-infante, Jose-luis; Brotons, Guillaume; Lagarde, Fabienne; Fabioux, Caroline; Soudant, Philippe; Huvet, Arnaud.
Marine plastic pollution is a major environmental issue. Given their ubiquitous nature and small dimensions, ingestion of microplastic (MP) and nanoplastic (NP) particles and their subsequent impact on marine life are a growing concern worldwide. Transfers along the trophic chain, including possible translocation, for which the hazards are less understood, are also a major preoccupation. Effects of MP ingestion have been studied on animals through laboratory exposure, showing impacts on feeding activity, reserve depletion and inflammatory responses, with consequences for fitness, notably reproduction. However, most experimental studies have used doses of manufactured virgin microspheres that may not be environmentally realistic. As for most...
Tipo: Text Palavras-chave: Microplastics; Nanoplastics; Experimental exposure; Impacts; Marine organisms; Physiology.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56088/57621.pdf
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Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis ArchiMer
Darias, Maria J.; Zambonino, Jose-luis; Hugot, K; Cahu, Chantal; Mazurais, David.
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were...
Tipo: Text Palavras-chave: Sea bass; Gene expression; Microarray; Development; Fish larvae.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4344.pdf
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FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax) ArchiMer
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
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Ano: 2010
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Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level ArchiMer
Politis, Sebastian Nikitas; Mazurais, David; Servili, Arianna; Zambonino-infante, Jose-luis; Miest, Joanna J.; Tomkiewicz, Jonna; Butts, Ian A. E..
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2 alpha, nkcc2 beta, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thr alpha A, thr alpha B, thr beta B, diol, dio2, dio3) were differentially expressed...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00448/55982/57456.pdf
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A first insight into genotype-diet interactions in European seabass (Dicentrarchus labrax) in the context of plant-based diet use ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont Nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00038/14907/12233.pdf
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Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae ArchiMer
Mazurais, David; Ernande, Bruno; Quazuguel, Patrick; Severe, Armelle; Huelvan, Christine; Madec, Lauriane; Mouchel, Olivier; Soudant, P.; Robbens, J.; Huvet, Arnaud; Zambonino-infante, J..
Microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. The present study aims at assessing the impact of polyethylene (PE 10-45µM) microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae. Fish were fed an inert diet including 0, 104 and 105 fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). Microbeads were detected in the gastrointestinal tract in all fish fed diet incorporating PE. Our data revealed an efficient elimination of PE beads from the gut since no fluorescent was observed in the larvae after 48h depuration. While the mortality rate increased significantly with the amount of microbeads scored per...
Tipo: Text Palavras-chave: Microplastic; Fish; Larvae; Ingestion; Survival.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00279/39057/37621.pdf
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A first insight into genotype x diet interactions in European sea bass (Dicentrarchus labrax L. 1756) in the context of plant-based diet use ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
This preliminary study assessed genotype x diet interaction in late growth of European sea bass (Dicentrarchus labrax) fed with either a fish meal (FM)- or a fish oil (FO)-based diet (M) or an all-plant-based (PB) diet. A total of 550 fish from 224 families were reared together and tagged. DNA was sampled and microsatellites were used to assign parentage. When fish weight was 192 +/- 54 g, two tanks were fed with M (FM: 100%; FO: 100%) and two others with PB (FM: 0% and FO: 0%). Body weight (BW), fork length (FL) and fillet lipid content (CorrFat) were analysed with a linear model and with REML methodology. We observed no significant differences between groups, but a slightly lower (P=0.03) daily growth coefficient in sea bass fed PB than in those fed M....
Tipo: Text Palavras-chave: Genotype by diet x environment interaction; European sea bass; Plant diet; Genetic correlation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14335/12374.pdf
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