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Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) International Journal of Morphology
Cavalcanti,George Maciel; Oliveira,André de Sá Braga; Assis,Thiago de Oliveira; Chimelli,Leila Maria Cardão; Medeiros,Paloma Lys de; Mota,Diógenes Luís da.
The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histochemistry was performed using myofibrillar ATPases (9.6, 4.6 and 4.3). To morphometrical analysis a computerized semiautomatic system and software Image-Lab was used. ATPase staining showed atrophy of muscle fibers. Fibrosis and adipose deposition occurred in variable degree depending of muscular involvement. The morphometrical analysis showed an increase of size (percentage) to type I fiber than other types in all patients. Furthermore, the type I fiber had a...
Tipo: Journal article Palavras-chave: Histochemistry analysis; Morphometric analysis; Vastus lateralis muscle; Duchenne muscular dystrophy.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046
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