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Gel filtration chromatography for the purification of protein and glucosidic molecules from phytopathogenic nematodes Sci. Agrar.Paran. / SAP
Gonçalves, Edilaine Della Valentina; Stangarlin, José Renato; Roncato, Sidiane Coltro; Dildey, Omari Dangelo Forlin; Meinerz, Cristiane Cláudia; Kuhn, Odair José.
- Female root-knot nematode can present compounds able to activate defense mechanisms in the vegetable. With this end, the chromatographic technique is based on the purification of molecules such as proteins, glycoproteins, nucleic acids and polysaccharides, which may present eliciting characteristics. The aim of this paper was to determine the elicitors purification methodology, from phytopathogenic nematodes, by gel filtration chromatography (GFC). Nematodes females of Meloidogyne incognita and Meloidogyne javanica were extracted, macerated, and subsequently subjected to gel filtration chromatography. For the M. incognita five protein peaks and seven glucosidic peaks, with molecular masses ranging from 8.80 to 285.95 KDa and from 65.09 to 7.47 µg glucose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meloidogyne incognita; Meloidogyne javanica; Eliciadores.
Ano: 2014 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11175
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Mycelial growth and production of microsclerotia of Macrophomina phaseolina confronted with different isolates of Trichoderma sp. Sci. Agrar.Paran. / SAP
Broetto, Laline; Coltro, Sidiane; Meinerz, Cristiane Cláudia; Dildey, Omari Dangelo Forlin; Pazdiora, Paulo César; Gonçalves, Edilaine Della Valentina; de Moares, Alice Jacobus; Henkemeir, Nicanor Pilarski; Kuhn, Odair José; Stangarlin, José Renato.
Charcoal Rot is a disease that occurs in a large number of plant species and besides not being economically controlled by fungicides no genetic resistance has been found. Associated with this controlling problem, there is also a growing awareness of the proper handling of pesticides or of the search for new and environmentally safer technologies for handling of plant diseases. Therefore this study aimed to examine in vitro the potential of different isolates of Trichoderma sp for biological control of Macrophomina phaseolina. Seventeen different isolates of Trichoderma sp. were used, in two different tests: direct confrontation with the pathogen and production of volatile compounds by the antagonist. In the experimental conditions the different isolates of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Controle biológico. Confronto direto. Composto volátil. Podridão Carvão..
Ano: 2014 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/7893
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