|
|
| Pérez,Edmundo A.; Fernández,Francisco J.; Fierro,Francisco; Mejía,Armando; Marcos,Ana T.; Martín,Juan F.; Barrios-González,Javier. |
| The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucose regulation; Glucokinase; Penicillin; Penicillium chrysogenum. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300017 |
| |
