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Borges,R.M.; Horne,J.H.; Melo,A.; Vidal,J.T.; Vieceli,F.M.; Melo,M.O.; Kanno,T.Y.N.; Fraser,S.E.; Yan,C.Y.I.. |
One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electroporation; In vivo gene expression; Electroporator; Vertebrate embryo. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900752 |
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Nora,D.B.; Gomes,I.; Said,G.; Carvalho,F.M.; Melo,A.. |
The long-term effects of low-level lead intoxication are not known. The sympathetic skin response (SSR) was evaluated in a group of 60 former workers of a primary lead smelter, located in Santo Amaro, BA, Brazil. The individuals participating in the study were submitted to a clinical-epidemiological evaluation including questions related to potential risk factors for intoxication, complaints related to peripheral nervous system (PNS) involvement, neurological clinical examination, and also to electromyography and nerve conduction studies and SSR evaluation. The sample consisted of 57 men and 3 women aged 34 to 69 years (mean ± SD: 46.8 ± 6.9). The neurophysiologic evaluation showed the presence of lumbosacral radiculopathy in one of the individuals (1.7%),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neuropathy; Sympathetic skin response; Lead intoxication; Peripheral nervous system. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100011 |
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