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| Germano,Mariana Gomes; Cannavan,Fabiana de Souza; Mendes,Lucas William; Lima,Amanda Barbosa; Teixeira,Wenceslau Geraldes; Pellizari,Vivian Helena; Tsai,Siu Mui. |
| The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catabolic genes; Genetic diversity; Land use systems; Microbial ecology; Tropical soils. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004 |
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| Navarrete,Acácio Aparecido; Taketani,Rodrigo Gouvêa; Mendes,Lucas William; Cannavan,Fabiana de Souza; Moreira,Fatima Maria de Souza; Tsai,Siu Mui. |
| The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soil microbial ecology; Soil microbiology; Microbial diversity; Land use changes; Tropical soils. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000500007 |
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