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| Zauli,Danielle Alves Gomes; Menezes,Carla Lisandre Paula de; Oliveira,Cristiane Lommez de; Mateo,Elvis Cristian Cueva; Ferreira,Alessandro Clayton de Souza. |
| Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QPCR; Hepatitis B; Hepatitis C. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400987 |
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