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Use of serological techniques for determination of Spodoptera frugiperda(J E Smith) predators (Lepidoptera: Noctuidae) Neotropical Entomology
Santos-Neto,Joaquim R; Mezencio,Jose M S; Chagas,Aline T A; Michereff-Filho,Miguel; Serrão,José E.
Spodoptera frugiperda (J E Smith) is an important pest of several crops, but especially on maize in Brazil. The implementation of biological control measures hinges on the identification of its predators and other natural enemies. As a means of identifying predators, antibodies against S. frugiperda eggs were generated by inoculating rabbits with macerated S. frugiperda eggs, and the production of antibodies against S. frugiperda egg proteins was verifi ed by double immunodiffusion (DID). These antibodies were then utilized in another serological technique, counterimmunoeletrophoresis (CIE), to identify insects that could have ingested S. frugiperda eggs. Macerates of entire insects collected in maize plantations and of individual parts of their digestive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fall armyworm; Natural enemy; Double immunodiffusion; Counterimmunoelectrophoresis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000300016
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