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| Conceição,Fabrício Rochedo; Michelon,André; Michelon,Marcelo; Cerqueira,Gustavo Maia de; Dellagostin,Odir Antonio. |
| Mycoplasmal pneumoniae is the main respiratory disease in swine. The most efficient way to control it is through the use of vaccines (bacterins), whose production cost is high. The objective of this work was to develop a new alternative for controlling Swine Mycoplasmal Pneumoniae, based on a recombinant subunit vaccine containing the R1 region of P97 adhesin of Mycoplasma hyopneumoniae fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (rLTB-R1). In this work we report the amplification of the genes, genetic fusion between LTB and R1 coding sequences, cloning, construction of the expression vector, as well as expression and purification of rLTB-R1 in E. coli. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine mycoplasmal pneumoniae; P97; LTB. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500027 |
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| Michelon,André; Michelon,Marcelo; Simionatto,Simone; Lagranha,Valeska Lizzi; Conceição,Fabrício Rochedo; Vaz,Eliana Knackfus; Cerqueira,Gustavo Maia; Dellagostin,Odir Antonio. |
| The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P<0.05) than the one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine colibacilosis; DNA vaccine; FaeC; Kozak sequence. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500029 |
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