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	Provedor de dados Genet. Mol. Biol. (1) Autor Almeida,Álvaro M. R. de (1) Freire,Maíra C. M. (1) Liebenberg,Merion M. (1) Mienie,Charlotte M. S. (1) Moreira,Maurilio A. (1) Oliveira,Luiz O. de (1) Schuster,Ivan (1) Mais... Palavra-chave Asian soybean rust (1) Diversity (1) Internal transcribed spacer (1) Phakopsora pachyrhizi (1) Phylogeography (1) Ano 2008 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA Genet. Mol. Biol. Freire,Maíra C. M.; Oliveira,Luiz O. de; Almeida,Álvaro M. R. de; Schuster,Ivan; Moreira,Maurilio A.; Liebenberg,Merion M.; Mienie,Charlotte M. S.. Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences... Palavras-chave: Phakopsora pachyrhizi; Diversity; Internal transcribed spacer; Asian soybean rust; Phylogeography. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020 Registros recuperados: 1 Primeira ... 1 ... Última

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