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| Milosevic,Snezana; Subotic,Angelina; Bulajic,Aleksandra; Djekic,Ivana; Jevremovic,Sladjana; Vucurovic,Ana; Krstic,Branka. |
| The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant... |
| Tipo: Journal article |
Palavras-chave: Meristem culture; New Guinea Impatiens; Tomato spotted wilt virus; Virus- free plants. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003 |
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