|
|
|
| Registros recuperados: 26 | |
|
|
| Kesarcodi-watson, Aditya; Miner, Philippe; Nicolas, Jean-louis; Asmani, Katia; Robert, Rene. |
| During a series of experiments, bacteriological elements in scallop larval rearing were investigated: larvae susceptibility to pathogens as a function of their age, and the use of probiotic bacteria during larviculture. Younger larvae (d5 PF) were highly more susceptible to pathogenic-challenge than their older siblings, which were challenged at an older age (d15 PF). A challenge with 104 CFU mL−1 of V. pectenicida killed 100% of d5 PF larvae 7 days following challenge, yet killed only 9% of d15 PF larvae 9 days following challenge. Use of the probiotics Phaeobacter gallaeciensis, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, provided for larger larvae, a high yield of competent larvae and, perhaps more importantly, protection against... |
| Tipo: Text |
Palavras-chave: Scallop; Pecten maximus; Probiotics; Alteromonas macleodii; Neptunomonas; Phaeobacter. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00210/32153/30900.pdf |
| |
|
| |
|
|
| Genard, Bertrand; Miner, Philippe; Nicolas, Jean-louis; Moraga, Dario; Boudry, Pierre; Pernet, Fabrice; Tremblay, Rejean. |
| Background: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase... |
| Tipo: Text |
|
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25504/23662.pdf |
| |
|
|
| Tremblay, Rejean; Cartier, Simon; Miner, Philippe; Pernet, Fabrice; Quere, Claudie; Moal, Jeanne; Muzellec, Marie-louise; Mazuret, Michel; Samain, Jean-francois. |
| The main objective of this study was to identify algal diets that maximize the survival and growth and alter the biochemical content of Pecten maximus larvae with the aim of improving metamorphosis. We also evaluated the potential of the cryptophyceae Rhodomonas salina as a food source for these larvae. Two flagellates, Isochrysis aff. galbana (T) and Pavlova lutheri (P), and two diatoms, Chaetoceros gracilis (C) or Skeletonema costatum (S), were tested as two ternary diets, namely PTC and PTS. PTC and PTS were compared with diets that also included R. salina (R). The addition of R. salina and the replacement of C. gracilis by S. costatum in the traditional hatchery diet seem to be two interesting alternatives for increasing the productivity of larval... |
| Tipo: Text |
Palavras-chave: Algal fatty acids and sterols; Energetic content; Metamorphosis; Nutrition; Scallop. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2404.pdf |
| |
|
|
| Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean. |
| The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival... |
| Tipo: Text |
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf |
| |
|
|
| Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline; Miner, Philippe; Suquet, Marc; Lambert, Christophe; Soudant, Philippe. |
| The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Sperm quality; Cellular parameters; Flow cytometry; Pacific oyster; Crassostrea gigas; Alexandrium catenella. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26853/24982.pdf |
| |
|
|
| Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
| Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
| Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
| |
|
|
| Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Fabioux, Caroline; Lambert, Christophe; Miner, Philippe; Le Grand, Jacqueline; Petton, Bruno; Hegaret, Helene. |
| Blooms of toxic dinoflagellates can co-occur with mass mortality events associated with herpesvirus OsHV-1 μVar infection that have been decimating Pacific oyster Crassostrea gigas spat and juveniles every summer since 2008 in France. This study investigated the possible effect of a harmful dinoflagellate, Alexandrium catenella, a producer of Paralytic Shellfish Toxins (PSTs), upon the oyster spat–herpesvirus interaction. Oyster spat from a hatchery were challenged by cohabitation with oysters contaminated in the field with OsHV-1 μVar and possibly other pathogens. Simultaneously, the oysters were exposed to cultured A. catenella. Infection with OsHV-1 μVar and PST accumulation were measured after 4 days of experimental exposure. Exposure to Alexandrium... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; OsHV-1 mu Var; Harmful algal blooms; Alexandrium; Host-pathogen interaction; PST accumulation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00269/38000/36101.pdf |
| |
|
|
| Harney, Ewan; Rastrick, Samuel; Artigaud, Sébastien; Pisapia, Julia; Miner, Philippe; Pichereau, Vianney; Strand, Oivind; Boudry, Pierre; Charrier, Gregory. |
| Elevated atmospheric CO2 is a major driver of global change in the ocean, causing sea surface temperatures to rise and acidification of marine environments. Together these stressors may have additive, synergistic or unforeseen consequences for many marine organisms, particularly during early life stages. The economically important King Scallop (Pecten maximus) is found along a large latitudinal gradient in the Eastern Atlantic, but it is unclear how this species will respond to changing environmental conditions. Studies of genetic structure suggest that Atlantic populations (from Spain to the UK) are genetically distinct from Norwegian populations, a result that is borne out by phenotypic differences. Yet whether genetic differences arise as a result of... |
| Tipo: Text |
|
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51520/52114.pdf |
| |
|
|
| Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Miner, Philippe; Le Grand, Jacqueline; Lambert, Christophe; Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline. |
| The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Alexandrium; Harmful algal blooms; Hemocyte; Microbial challenge. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00314/42523/41894.pdf |
| |
|
|
| Seguineau, Catherine; Soudant, P; Moal, Jeanne; Delaporte, Maryse; Miner, Philippe; Quere, Claudie; Samain, Jean-francois. |
| The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and... |
| Tipo: Text |
Palavras-chave: Food supply; Arachidonic acid; Spat; Crassostrea gigas; Dietary. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1094.pdf |
| |
|
|
| Toullec, Jordan; Vincent, Dorothée; Frohn, Laura; Miner, Philippe; Le Goff, Manon; Devesa, Jérémy; Moriceau, Brivaela. |
| In marine ecosystems, carbon export is driven by particle flux which is modulated by aggregation, remineralization, and grazing processes. Zooplankton contribute to the sinking flux through the egestion of fast sinking fecal pellets but may also attenuate the flux by tearing apart phytoplankton aggregates into small pieces through swimming activity or direct ingestion. Freely suspended cells, artificial monospecific aggregates from two different diatom species (Chaetoceros neogracile and Skeletonema marinoi) and natural aggregates of Melosira sp. were independently incubated with five different copepod species (Acartia clausi, Temora longicornis, Calanus helgolandicus, Euterpina acutifrons, and Calanus hyperboreus). During the grazing experiments initiated... |
| Tipo: Text |
Palavras-chave: Diatom aggregate; Grazing experiment; Copepod; Sinking velocity; Particle dynamics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00594/70605/68799.pdf |
| |
|
|
| Dao, Jean-claude; Barret, Jean; Carval, Jean-pierre; Connan, Jean-paul; Corre, Christian; Devauchelle, Nicole; Fleury, Pierre-gildas; Laurent, Pascale; Mazuret, Michel; Miner, Philippe; Mingant, Christian; Muzellec, Marie-louise; Robert, Rene. |
| Aquaculture production of scallop has been identified by a series of technical operations during the past ten years programme under a partnership involving the fishermen organizations with regional and national grants. 3 million juveniles have been produced annually, giving a potential recapture by fishing of 100 to 120 tonnes 3 years later. Results in terms of technical and economical approach have pointed out the principal unknown factors responsable for high costs. 7he pilot programme aims to increase the production to reach a level of 5 million juveniles to support the fishery of the rade de Brest and induce new management of the resources. Two priorities were selected, control of the reproduction in the hatchery to obtain reliable larval and... |
| Tipo: Text |
|
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00044/15494/12860.pdf |
| |
|
|
| Robert, Rene; Trut, Gilles; Laborde, Jean-louis; Miner, Philippe. |
| Until 1960, the black scallop Chlamys varia (Linné, 1758) was largely distributed over the French Atlantic coast. Nowadays, most of the deposits are gone. Since controlled mollusc reproduction techniques are now relatively mastered, some reimplatation attempts have been conducted on a number of oyster culture sites, including the Arcachon Bay, from 1989 to 1992. Despite some good results in hatchery and nursery, the rearing attempts failed. If some good growth rates have been monitored in the Arcachon Bay over these three years, these performances have been frustrated by very high mortality rates occurring shortly after the setting up of the farms. Although this species is known to be relatively sensitive to environmental factors, the hydrobiological... |
| Tipo: Text |
Palavras-chave: Bassin Arcachon; Elevage; Chlamys varia; Pétoncle. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-6193.PDF |
| |
|
|
| Gagne, Renee; Tremblay, Rejean; Olivier, Frederic; Pernet, Fabrice; Miner, Philippe; Samain, Jean-francois. |
| Through biological flume experiments, we studied the effect of shear velocity and attachment period on the percent detachment of Pecten maximus post-larvae of different sizes reared on two experimental diets. The first diet, (Pavlova lutheri, Isochrysis galbana, Chaetoceros calcitrans), which served as a reference diet (PTC), is commonly used in bivalve hatcheries; the second is the PTC diet with the addition of Rhodomonas salina (PTCR). Our results indicate that the detachment of post-larvae fed PTCR diet showed significant interactions between scallop length, attachment period and shear stress, while the post-larvae feed with the PTC diet showed only effect of shear stress factor. Furthermore, post-larvae exposed to various flume velocities showed... |
| Tipo: Text |
Palavras-chave: Attachment; Pecten maximus; Benthic boundary layer; Flume; Diet. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00111/22219/20056.pdf |
| |
|
|
| Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Bideau, Antoine; Le Goic, Nelly; Fleury, Elodie; Miner, Philippe; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
| The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6... |
| Tipo: Text |
Palavras-chave: Synthesis pathway; Diatom; 20:5n-3 (EPA); Chaetoceros muelleri; Acyl-editing mechanism; Compound-specific isotope analysis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74257/73887.pdf |
| |
|
|
| Pauletto, Marianna; Di Camillo, Barbara; Miner, Philippe; Huvet, Arnaud; Quillien, Virgile; Milan, Massimo; Ferraresso, Serena; Pegolo, Sara; Patarnello, Tomaso; Bargelloni, Luca. |
| Among reared bivalves, some “novel species”, such as the great scallop, Pecten maximus, have experienced more difficulty with routine reproduction due to their high sensitivity to biological, chemical, and physical stress during stages of early development. Working with high larval densities requires the use of aeration systems to provide optimal larval suspension and feed distribution. The high susceptibility of the great scallop to aeration in small-volume systems may impose an important limitation in hatchery-based practices. The present study aimed to investigate the processes impacted by aeration in P. maximus veliger larvae exposed to continuous aeration in small-volume tanks (10 L). Aeration appeared as major stressor that was responsible for early... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56420/58327.pdf |
| |
|
|
| Harney, Ewan; Artigaud, Sebastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia. |
| Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 °C), acidified (pH 7.9, 20 °C), warm (pH 8.1, 22 °C) or warm acidified (pH 7.9, 20 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Ocean acidification; Larval development; ATP synthase; GAPDH; Superoxide dismutase. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00299/40985/40076.pdf |
| |
|
|
| Offret, Clement; Paulino, Sauvann; Gauthier, Olivier; Château, Kevin; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Pernet, Fabrice; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaelle Le. |
| Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position.... |
| Tipo: Text |
Palavras-chave: Microbiota; Oysters; Clams; Intertidal zone; Digestive gland; Metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/73961/73320.pdf |
| |
|
|
| Robert, Rene; Miner, Philippe; Mazuret, Michel; Connan, Jean-paul. |
| The influence of density on Patinopecten yessoensis larval development was studied during production of an F2 generation. Setting behaviour of P. yessoensis on PVC removable spat collectors, previously tested with Pecten maximus, was determined. Poor larval growth of P. yessoensis was observed at a larval density of 7 ·ml·1 and post larval settlement occurred mainly on the bottom of the baskets, as has been observed with the king scallop, P. maximus. The PVC collector is not suitable for either species of scallop. |
| Tipo: Text |
|
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00090/20163/17816.pdf |
| |
| Registros recuperados: 26 | |
|
|
|
