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Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk BJM
Cirolini,Andréia; Miotto,Marília; Machado,Fernanda Morgana; Silva,Helen Silvestre da; Ogliari,Paulo José; Vieira,Cleide Rosana Werneck.
The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Milk; Alternative methods; ISO 21528-2:2004; Count.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300016
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Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk Ciênc. Tecnol. Aliment.
Cirolini,Andréia; Baseggio,Andressa Mara; Miotto,Marília; Ramos,Roberta Juliano; Cattani,Cristhiane Stecanella de Oliveira; Vieira,Cleide Rosana Werneck.
New microbiological methods have been developed and commercialized, but their performance must be guaranteed. The aim of the present study was to evaluate the PetrifilmTM and TEMPO® systems compared to the conventional method for counting microorganisms in pasteurized milk. A total of 141 samples of pasteurized milk were analyzed by counting mesophilic aerobic, Coliforms at 35 ºC, Coliforms at 45 ºC, and Escherichia coli microorganisms. High correlation was found between the methods for counting Coliforms at 35 ºC, but low correlation was found for counting mesophilic aerobic, Coliforms at 45 ºC, and Escherichia coli. No significant statistical difference was found among the three methods for counting Coliforms at 35 ºC; however, the mean counts of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternative methods; Milk; Microbial contamination; Conventional methods.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400026
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