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Mojsov, Kiro. |
The paper examines effects of different inorganic salts in an apple pulp base on the production of pectinolytic enzymes with the aim of optimizing the medium for maximal enzyme production. The apple pulp combined with corn flour and simple mineral salts was used as a nourishing base in submerged production of pectinolytic enzymes by the fungus Aspergillus niger MK-15. The growth of the microorganism (dry weight) on different sources of nitrogen showed maximum dry weight with (NH4)2HPO4. The growth of the microorganism (dry weight) on different concentration of (NH4)2HPO4 (by 0.2% to 0.8%) provided maximal dry weight with 0.7% (NH4)2HPO4. The different inorganic salts (sources of nitrogen) on base stimulated the production of pectinolytic enzymes and... |
Tipo: Journal Article |
Palavras-chave: Apple pulp; Fermentation; Inorganic salts; Pectinolytic enzymes; Aspergillus niger; Agribusiness; Research and Development/Tech Change/Emerging Technologies; L65. |
Ano: 2010 |
URL: http://purl.umn.edu/94732 |
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Mojsov, Kiro. |
For the purpose of acquiring a highly active producer of pectolytic enzymes, there was a probe of isolating 140 species of Aspergillus’s fungi from different substrates (soils, grape malts, grapes, apples and sugar beet shreds). The isolated kinds of fungi were kept on a slant agar surface according to Chapek, with 2% of pectin. Testing was performed on the isolated layers of fungi as to the production of pectinolytic enzymes. The nourishing base used was the synthetic Chapek base with 2% pectin, 2% lactose and 0.7 % (NH4)2HPO4, and a natural base of 1% refuse apple pulp. Within 48 hours after cultivating the fungi, the filtrates were tested by the viscozimetric method to determine their entire pectolytic activity. The acquired results showed that a... |
Tipo: Journal Article |
Palavras-chave: Biosynthesis; Pectolytic enzymes; Apple pulp; Aspergillus.; Land Economics/Use; L65. |
Ano: 2009 |
URL: http://purl.umn.edu/94608 |
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