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| Osorio,Mayra; Gámez,Elizabeth; Molina,Sandy; Infante,Diógenes. |
| Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the... |
| Tipo: Journal article |
Palavras-chave: AFLP; Genetic stability; ISTR; SSR; UPGMA. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003 |
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| Sosa,Daynet; Pérez Martínez,Simón; Molina,Sandy; Demey,Jhonny; Gómez,Kelly; Domínguez,Diamarys; Istúriz,María; Rumbos,Raisa; Parra,Dercy. |
| Se estudió la diversidad genética entre 68 aislados de Trichoderma procedentes de diferentes cultivos, sustratos y localidades, mediante los análisis ITS-AFLP. Se secuenció la región ITS1-5.8S-ITS2 del ADN ribosomal amplificada con los cebadores ITS1 e ITS4 y en el caso de los AFLP se utilizaron cuatro combinaciones de oligonucleótidos. Las relaciones genéticas entre los aislados se analizaron mediante el uso combinado del Análisis de Coordenadas Principales, el Análisis de Conglomerados y el ajuste de un Biplot Logístico Externo sobre datos de disimilitud, utilizando los coeficientes de Jaccard, Emparejamiento simple, Dice y Rogers y Tanimoto. Se identificaron nueve especies y las más abundantes fueron Hypocrea lixii (anamorfoTrichoderma harzianum) y T.... |
| Tipo: Journal article |
Palavras-chave: Trichoderma; ITS; AFLP; Polimorfismo; Control biológico. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100005 |
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