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Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus ) Neotropical Ichthyology
Copatti,Carlos Eduardo; Baldisserotto,Bernardo; Souza,Carine de Freitas; Monserrat,José María; Garcia,Luciano.
ABSTRACT This study aimed to assess the effects of low and high water hardness in interaction with different water pH in pacu (Piaractus mesopotamicus). Pacu juveniles were subjected to low (50 mg CaCO3 L-1 - LWH) or high water hardness (120 mg CaCO3 L-1 - HWH) at water pH of 5.5 (acidic), 7.5 (circumneutral) or 9.0 (alkaline) for 15 days. Gills and kidneys were collected (days 1, 5 and 15). Gill Na+/K+-ATPase (NKA) and vacuolar-type H+-ATPase (V-ATPase) activities were higher in alkaline pH with HWH on day 1. Gill and kidney NKA and V-ATPase activities were higher in acidic pH with LWH on day 15. Gill NKA activity of pacus under alkaline pH with LWH was higher than those exposed to HWH. Reduced antioxidant capacity in the gills and kidney and enhanced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acidic pH; Alkaline pH; GST; Na+/K+-ATPase; TBARS.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500204
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Growth, antioxidant system, and immunological status of shrimp in bioflocs and clear water culture systems PAB
Souza,Diego Moreira de; Kutter,Mateus Tavares; Furtado,Plinio Schmidt; Romano,Luis Alberto; Wasielesky Junior,Wilson; Monserrat,José María; Garcia,Luciano.
Abstract: The objective of this work was to evaluate the effect of the traditional culture system in clear water and of the one in microbial flocs on the antioxidant and immunological status of Litopenaeus vannamei shrimp. Samples (gill, hemolymph, and hepatopancreas) were collected on days 15, 30, and 60 of the experimental period. The following immunological parameters were analyzed at each evaluation time: granular and hyaline hemocytes, total protein, and apoptosis. Assays on glutamate-cysteine ligase (GCL) activity and on the concentrations of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were also performed. The evaluated immunological parameters did not differ significantly between treatments. Shrimp reared in clear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; BFT; Oxidative stress; TBARS.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000101100
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