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| Sousa,Diego Rayan Teixeira de; Santos,Carla Silvana da Silva; Wanke,Bodo; Silva Júnior,Roberto Moreira da; Santos,Marcelo Cordeiro dos; Cruz,Kátia Santana; Monte,Rossicléia Lins; Nocker,Andreas; Souza,João Vicente Braga de. |
| Background The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, microscopy, and biochemical testing). Results The assays carried out with the reference strains (Candida albicans, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus gattii and Histoplasma capsulatum), demonstrated that the RFLP profiles were correctly predicted by the in silico... |
| Tipo: Journal article |
Palavras-chave: Pathogenic fungus; Polymorphism; Simultaneous analysis. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300014 |
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| Silva,Leila Cristina Ferreira; Miranda,Angélica Espinosa; Batalha,Rosieny Santos; Monte,Rossicléia Lins; Talhari,Sinésio. |
| OBJECTIVES: Our goal was to determine the prevalence of Trichomonas vaginalis and its associated factors among women living with HIV attending an AIDS clinic in Manaus, Amazonas, Brazil. METHODS: Cross-sectional study among women attending an AIDS clinic in Manaus between March and December 2010 for gynecological examination were invited to participate. Enrolled patients answered a face-to-face interview including demographic, behavioral and clinical data. They also underwent a gynecological evaluation and cervical scrape samples were collected for wet mount, Gram stain, culture and cytological analysis. A blood sample was obtained to determine TCD4+ lymphocytes and viral load. RESULTS: A total of 341 (91.2%) women participated in the study. The prevalence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichomonas vaginalis; HIV; Associated factors; Amazonas. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600012 |
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