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BIOPROSPECTING: A TOOL TO CONSERVE CHILEAN BRYOPHYTES Gayana Botánica
Cuvertino-Santoni,Jorge; Montenegro,Gloria.
Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,...
Tipo: Journal article Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002
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RELACION ENTRE EL ORIGEN FLORAL Y EL PERFIL DE ELEMENTOS MINERALES EN MIELES CHILENAS Gayana Botánica
Montenegro,Gloria; Fredes,Carolina.
In this report, 61 honey samples were collected in beehives located between IV and X Administrative Region of Chile. We focussed the analysis in risky chemical elements for human health and in the main chemical elements found previously in Chilean honeys. The analysis of Al, Sr, Cu, Fe, Mn and Zn were done by wet acid digestion method by ICP-OES. Lotus pedunculatus and Eucryphia cordifolia honeys showed the highest Al contents related to their geographical origin (Bío-Bío and Los Lagos Regions). The principal component analysis did not show groups of honeys with the some floral origin and a typical chemical element profile.
Tipo: Journal article
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432008000100014
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El Propóleos Reduce la Esteatosis Hepática Inducida por Dieta en Ratones International Journal of Morphology
Herrera,Christian L; Fritz,Osvaldo; Montenegro,Gloria; Alvear,Marysol; del Sol,Mariano; Salazar,Luis A.
El hígado graso no alcohólico (HGNA), comprende un amplio espectro de lesiones, que van desde esteatosis hepática, hasta cirrosis y carcinoma hepatocelular, siendo sus principales factores de riesgo los desordenes asociados a síndrome metabólico (SM). El propóleos, sustancias resinosa elaborada por Apis mellifera para la protección de la colmena, ha demostrado un efecto hepatoprotector, así el objetivo de esta investigación fue evaluar el efecto de un propóleos chileno sobre el desarrollo de esteatosis hepática no alcohólica en ratones C57BL/6J sometidos a una dieta aterogénica tipo Paigen. Veintiocho ratones (C57BL/6J), divididos en: 1, dieta balanceada (CH); 2, dieta hipercolesterolémica (HC); 3, dieta HC + 10 mg/kg/día de propóleos (BP); 4, dieta HC +...
Tipo: Journal article Palavras-chave: Esteatosis hepática; HGNA; Dieta aterogénica; Propóleos.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100010
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Multivariate analysis of pollen frequency of the native species Escallonia pulverulenta (Saxifragaceae) in Chilean honeys Rev. Bras. Bot.
Montenegro,Gloria; Peña,Raúl C.; Pizarro,Rodrigo.
The aim of this work was the identification of geographic zones suitable for the production of honeys in which pollen grains of Escallonia pulverulenta (Ruiz & Pav.) Pers. (Saxifragaceae) can be detected. The analysis of botanical origin of 240 honey samples produced between La Serena and Puerto Mont (the IV and X Administrative Regions of Chile), allowed the detection of pollen grains of E. pulverulenta in 46 Chilean honeys. The geographic distribution of the honeys studied is presented together with their affinities, through factor analysis and frequency tables. The study was based on the presence of E. pulverulenta pollen. Escallonia pulverulenta pollen percentages oscillated between 0.24% and 78.5%. Seventeen of the studied samples were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Chile; Escallonia; Mediterranean type climates; Unifloral honey.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042010000400010
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Contenidos de metales pesados en suelos cercanos a un relave cuprífero Chileno Agrociencia
Montenegro,Gloria; Fredes,Carolina; Mejías,Enrique; Bonomelli,Claudia; Olivares,Luis.
En Chile, la contaminación por metales pesados se produce principalmente cerca de las explotaciones de la minería cuprífera en las regiones del norte y centro del país. En éstas se han encontrado suelos agrícolas contaminados por metales pesados ubicados a varios kilómetros de la fuente de contaminación. El objetivo de este estudio fue analizar los contenidos de Fe, Cu, Mn, Zn, Cr, Cd y Pb y variables químicas (pH, CE, MO y CIC) de suelos cercanos a un relave de Cu ubicado en el norte del país. La determinación de metales pesados disponibles se realizó mediante AAS, y los metales pesados totales mediante ICP-OES. Los suelos fueron agrupados en tres sectores de acuerdo a la cercanía del relave, el curso de un estero y el sentido del viento. El Cu fue el...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Análisis de componentes principales y suelos contaminados; Cobre; Metales pesados.
Ano: 2009 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952009000400009
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Phenolic profiles of nectar and honey of Quillaja saponaria Mol. (Quillajaceae) as potential chemical markers Biol. Res.
Montenegro,Gloria; Díaz-Forestier,Javiera; Fredes,Carolina; Rodríguez,Sharon.
Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection) showed a similar profile of phenolic...
Tipo: Journal article Palavras-chave: Apis mellifera; Chromatographic profile; Floral nectar; Honey; Phenolic compounds; Quillaja saponaria.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200009
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Biological applications of honeys produced by Apis mellifera Biol. Res.
Montenegro,Gloria; Mejías,Enrique.
Honey is a natural product with many attributes that are useful for humans. The consumption of honey is increasing because of its beneficial biological properties, including antioxidant and antibacterial activities. Even though honey is produced worldwide, many variants of this product have not been studied and their biological potential for alternative uses has not been evaluated. Because of its varied endemic native flora, Chile produces several types of honey. It is likely that these apian products have important biological properties inherited from specific floral sources, but it is absolutely necessary to conduct further chemical analyses to identify and characterize these biological attributes. Sadly, the potential antimicrobial and antitumor...
Tipo: Journal article Palavras-chave: Honeys; Antioxidants; Natural Antibiotics; Environmental Bioindicator.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400005
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Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus BJM
Barrientos,Leticia; Herrera,Christian L.; Montenegro,Gloria; Ortega,Ximena; Veloz,Jorge; Alvear,Marysol; Cuevas,Alejandro; Saavedra,Nicolás; Salazar,Luis A..
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Botanical characterization; Chemical characterization; Streptococcus mutans; Streptococcus sobrinus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038
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The antifungal effect of six commercial extracts of Chilean propolis on Candida spp Ciencia e Investigación Agraria
Herrera,Christian L; Alvear,Marysol; Barrientos,Leticia; Montenegro,Gloria; Salazar,Luis A.
Propolis has been used in traditional medicine for many centuries because of its benefcial health properties, including its antimicrobial capacity. Prosthesis stomatitis affects a signifcant percentage of users of removable dentures; Candida albicans is the most common fungal species associated with the development of this pathology. Thus, the objectives of this study were: a. To evaluate the antifungal activity of six commercial propolis extracts against Candida spp. that was isolated from the oral cavity of removable dentures users, and b. To determine chemical characteristics of the propolis extracts evaluated. Among the results, we note that these concentrations of polyphenols varied between 9 ± 0.3 and 85 ± 2.1 mg mL-1. Chromatographic analysis was...
Tipo: Journal article Palavras-chave: Antifungal activity; Candida albicans; Propolis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000100007
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Effect of fire on herbaceous "matorral" vegetation of Central Chile Ciencia e Investigación Agraria
Sabadin,Patricia; Gómez,Miguel; Ginocchio,Rosanna; Peña,Iván; Mujica,Ana María; Montenegro,Gloria.
This research aimed to determine the diversity and biomass of species in burned in the Jardín Botánico Nacional of Viña del Mar (JBN), region of Valparaíso, Chile (Lat. S 33° 02’ and Long. 71° 35’ O) from April to December 2012. Three sites were selected that were located on slopes with the same exposure: 1) site burned by fire in 2012, 2) site burned by fire in 2003, and 3) control site corresponding to an area without previous fires. Both shrub and herbaceous species were studied. The cover of shrubs, geophytes and therophytes was determined as well as the aboveground and belowground biomass of geophytes, with special attention paid to Oziroe arida. When O. arida was present, the diameter of its bulbs were studied. All information was...
Tipo: Journal article Palavras-chave: Aboveground biomass; Belowground biomass; Cover; Fires; Geophytes; Oziroe arida; Shrub species; Therophytes.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000300010
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The medicinal value of honey: a review on its benefits to human health, with a special focus on its effects on glycemic regulation Ciencia e Investigación Agraria
Cortés,Manuel E; Vigil,Pilar; Montenegro,Gloria.
Honey, a natural substance produced by honeybees, is composed of a complex mixture of carbohydrates, water, and a small amount of proteins, vitamins, minerals, and phenolic compounds. Fructose, glucose and maltose are among the various types of sugars present in honey. Used for millennia as both food and medicine, honey has been associated with improved antioxidant capacity, modulation of the immune system, antimicrobial activities, influence on lipid values (through antihypercholesterolemic effects) and regulation of glycemic responses, among other benefits. The aim of this article was to review the effects of natural honey intake on human health, with particular reference to its influence on glycemic regulation. Several studies have focused on the...
Tipo: Journal article Palavras-chave: Diabetes; Flavonoids; Glycemic regulation; Honey; Insulin resistance; Nutraceuticals; Nutrition.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000200015
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Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum Ciencia e Investigación Agraria
Saavedra,Nicolás; Barrientos,Leticia; Herrera,Christian L; Alvear,Marysol; Montenegro,Gloria; Salazar,Luis A.
Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after...
Tipo: Journal article Palavras-chave: Lactobacillus fermentum; Dental caries; Propolis; Antibacterial activity.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011
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Genetically modified crops in Chile Ciencia e Investigación Agraria
Salazar,Erika; Montenegro,Gloria.
The economical, environmental and social impacts associated with genetically modified (GM) crops are supported by the increased use of GM species by farmers. This increase in the use of GM species has included a global increase in both the number of hectares with GM crops and the number of countries using this technology. However, the use of GM crops has some drawbacks, due to the environmental risks associated with some of the events and social risks related to intellectual property rights issues and the monopoly associated with this technology. An overview of the current status of the use of GM crops in relation to the production, benefits, associated risks, intellectual property rights, legislation and regulatory framework is presented, focusing in the...
Tipo: Journal article Palavras-chave: Genetically modified organisms; Regulatory framework; Intellectual property; Chile.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000300003
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Propagation of Sophora toromiro through interspecific grafting to support species conservation: Masificación de Sophora toromiro, mediante injerto inter específico como apoyo a la conservación de la especie Ciencia e Investigación Agraria
Espejo,Jaime; Baeza,Marcelo; Ruiz,Eduardo; Mora,Freddy; Gomez,Miguel; Montenegro,Gloria.
This report provides information on applying the cleft grafting technique in the species Sophora toromiro (Phil.) Skottsb., focused on its propagation, and contributes to the conservation of the genetic base of this species in the medium and long term. A germplasm collection was obtained via interspecific grafting using Sophora cassioides (Phil.) Sparre as rootstock, employing two variations of cleft grafting sealing. The survival of 301 S. toromiro grafts of the Titze line at ten months was 64% or 95%, according to the type of sealing performed. A second experiment, comparing the origin of the material, indicated that the plants of Goteborg origin showed survival values close to 63%, versus 58% for those from the Jardín Botánico Nacional de Viña del Mar...
Tipo: Journal article Palavras-chave: Conservation; Graft; Sophora toromiro; S. cassioides.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100019
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Nutritíonal Composition and Polyphenolic Content of Edible Peumus boldus Mol. Fruits Ciencia e Investigación Agraria
Velásquez,Patricia; Sandoval,María Ignacia; Giordano,Ady; Gómez,Miguel; Montenegro,Gloria.
Peumus boldus is an endemic Chilean tree whose edible fruit lack a history of scientific research. The aim of this study was to determine its nutritional composition (moisture, ash, crude fiber, proteins, carbohydrates and lipids) and polyphenolic content (total phenolic content, HPLC-DAD, HPLC-MS/MS analysis) at different stages of development. The results show that the fruit decreased in ash, crude fiber and total phenolic content throughout its development, while its carbohydrate and protein content increased. An increase in phenolic molecules such as catechin and epicatechin was also observed. This is the first report on the nutritional composition and phenolic content of Peumus boldus Mol. fruits.
Tipo: Journal article Palavras-chave: Ash; Crude fiber; Kjeldahl; Goldfish; Phenol-sulfuric method; Total phenolic content.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100007
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Pathogen control using a natural Chilean bee pollen extract of known botanical origin Ciencia e Investigación Agraria
Cabrera,Carol; Montenegro,Gloria.
Bee pollen is a product from beehives that is contained in corbiculae, generally monospecific and is currently considered as a functional food due to its nutritional and medicinal properties. This study investigates the antimicrobial activity of a Chilean bee pollen extract of known botanical origin. The botanical origin of the pollen was determined by palynological analysis, and then an aqueous extract was prepared. The antibacterial properties of the extract were evaluated on human infectious agents (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes) using a qualitative method (agar diffusion) and a quantitative method (minimum inhibitory and bactericide concentration) and on agricultural pathogens (Alternaria...
Tipo: Journal article Palavras-chave: Antimicrobial activity; Bee pollen; Aqueous extract.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100020
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Identification and characterization of an original grapevine cultivar (Vitis vinifera) found in Chile Ciencia e Investigación Agraria
Gonzalez,Alvaro S; Massera,Franco; Moscoso,Daniel; Hinrichsen,Patricio; Montenegro,Gloria; Laucou,Valerie; Lacombe,Thierry; Boursiquot,Jean-Michel; Pszczólkowski,Philippo.
Currently, many monovarietal vineyards present a minority of mixed cultivars. Some of these cultivars are unknown and could represent an opportunity to discover new and potentially useful genotypes both for research and production purposes. In a 'Carmenère' vineyard planted in 1994 in Palmilla (Colchagua Valley, Chile), a minority presence of other cultivars was found. The present work covers for the first time the identification and characterization of one of these cultivars, which presented a very poor fruit set. Through classic ampelography, it was not possible to associate the studied cultivar with any known cultivar in Chile. However, through a simple sequence repeats (SSR) comparison against the database of the INRA Germplasm Repository "Domaine de...
Tipo: Journal article Palavras-chave: Ampelography; Chile; Coulure; Molecular markers; Poor fruit set; SSR; Triploid.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200016
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Aplicación de la Norma Chilena Oficial de denominación de origen botánico de la miel para la caracterización de la producción apícola Ciencia e Investigación Agraria
Montenegro,Gloria; Gómez,Miguel; Díaz-Forestier,Javiera; Pizarro,Rodrigo.
La producción apícola chilena se caracteriza por una gran variedad de tipos de mieles, con una alta participación de especies vegetales nativas, las que les otorgan cualidades particulares, debido al alto endemismo de su flora. Alrededor del 95% de la miel producida en Chile es exportada a granel, sin ningún valor agregado, lo que equivale al 1% de participación en el mercado de exportación melífera. Como es imposible tener alguna ingerencia en el comercio mundial de este producto, se hace necesario mejorar la competitividad diferenciando la producción y convirtiéndola en un producto particular, con características únicas. Un avance importante en este sentido ha sido el establecimiento de un sistema de trazabilidad y la implementación de la Norma Chilena...
Tipo: Journal article Palavras-chave: Abeja; Miel; Origen botánico; Tipificación; Mieles chilenas.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000200007
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Comparison of methods for determining the color of Chilean honeys and the relationship of color with botanical origin in central Chile Ciencia e Investigación Agraria
Martin,María José; Fredes,Carolina; Nuñez,Gabriel; Ginocchio,Rosanna; Montenegro,Gloria.
The color of honey has special relevance in the international market, as different markets demand specific colors of honey. Chile has very diverse native and exotic flora, which are used by bees (Apis mellifera) to produce a range of honey types, including honeys of different colors. In this context, determining the relationships between color and botanical origin among Chilean honeys is very relevant. The objectives of the present study were to compare three methodologies for determining color in Chilean honeys (Pantone®, optical density (OD), and Pfund) and to define the relationship between color and botanical origin of the characterized honeys. The most common honeys from the Libertador Bernardo O'Higgins Region (VI Region) of Chile were Galega...
Tipo: Journal article Palavras-chave: Honey markets; Melissopalynological analysis; Optical density; Pantone®; Pfund.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000300014
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Determination of suitable zones for apitourism using multi-criteria evaluation in geographic information systems: a case study in the O'Higgins Region, Chile Ciencia e Investigación Agraria
Pantoja,Gabriel; Gómez,Miguel; Contreras,Claudio; Grimau,Lissette; Montenegro,Gloria.
Abstract Beekeeping, which involves honey production and pollination of agricultural crops, has become a significant production activity. Linking beekeeping with ecotourism brings greater value to the beekeeping sector, thereby attracting the development of apitourism. Multi-criteria decision analysis is a set of techniques developed through Geographic Information Systems (GIS) focused on spatial decision making, which can be used to link beekeeping and tourism for the development of priority sites for shared activity. This work is based on the definition and weighting of factors used for evaluation of apitourism suitability in Chile, considering the opinion of beekeepers and professionals associated with the sector. The result is a map of apitouristic...
Tipo: Journal article Palavras-chave: Beekeeping; Geographic information systems (GIS); Land-use suitability analysis; Melliferous plant; Multi-criteria decision analysis (MCDA); Tourism.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200139
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